• Sucheewan Yoyrurob
  • Orasa Naban
  • Supattra Choopia
  • Boonya Charnnok
  • Palakorn Boonsai




Methane, Ethanol pretreatment, Batch digestion, Oil palm fiber


Oil Palm Fiber (OPF) is an interesting lignocellulose biomass but the limitation is the lignin component. It is difficult to decompose in anaerobic conditions for biogas production. This research aimed to study the effect of ethanol pretreatment (10-30% of ethanol) on the potential of methane production from oil palm fiber. Palm fiber pretreatment was performed at 190°C for 30 minutes. The results showed that the optimum concentration in ethanol pretreatment to enhance the potential methane from the palm fiber was 50% due to lignin was highly soluble in ethanol then the structure of pretreated OPF to be in optimum condition for fermentation. This pretreatment condition provided the highest potential methane of 161.8+6.2 LCH4/kgVS which accounted for 52.2% higher than untreated oil palm fiber. In addition, this pretreatment condition can increase the potential of methane production by 2.1 times. Finally, valorizing lignin and recycling ethanol should be performed to achieve economic feasibility, reduce waste and be more environmentally friendly.


Boonsai P, Charnnok B, Yoyrurob S. Improvement of digestibility of methane production from oil palm fiber by hydrothermal pretreatment. Life Sciences and Environment Journal 2020;21(2):311-321.

Chaipa S, Asavavisthchai S. Biogas production from palm fiber and empty fruit bunch by using sludge from anaerobic wastewater treatment system. Proceeding of the 13th KU-KPS Conference; 2016.

Charnnok B, Sawangkeaw R, Chaiprapat S. Integrated process for the production of fermentable sugar and methane from rubber wood. Bioresource Technology 2020;302:122785.

Dechrugsa S, Kantachote D, Chaiprapat S. Effect of inoculum to substrate ratio, substrate mix ratio and inoculum source on batch co-digestion of grass and pig manure. Bioresource Technology 2013; 146:101-108.

Jungniyom T. Zero-waste process in oil palm extraction industries. Hatyai Academic Journal 2008; 6(2):159-164.

Kongdang C, Suksaroj C, Chungsiriporn J. Biogas production by anaerobic batch co-digestion of pig manure and rubber leaves. Proceeding of the 5th PSU-UNS International Conference on Engineering and Technology (ICET-2011), Phuket; 2011, 114-118.

McCarty PL. Anaerobic waste treatment fundamentals-part two: environmental requirements and control. Public Works; 1964.

Ministry of energy. Alternative Energy Development Plan: AEDP2015. 2015. Available at: http://www.eppo.go.th/ images/policy/pdf/aedp2015.pdf. Accessed March 3, 2020.

Noparat P. Organosolv pretreatment of oil palm trunk for enzymatic hydrolysis. The Journal of Applied Science 2018;17(1):75-86.

O-thong, Boe K, Angelidaki I. Thermophilic anaerobic co-digestion of oil palm empty fruit bunches with palm oil mill effluent for efficient biogas. Applied Energy 2012;93:648-654.

Piakong P. Study of xylose production from oil palm empty fruit bunch. Master of Engineering in Chemical Engineering, Prince of Songkla University; 2014.

Polprasert S. Pretreatment of lignocellulosic materials for ethanol production. Thai Science and Technology Journal 2014;22(5):641-649.

Roy R, Rahman MS, Raynie DE. Recent advances of greener pretreatment technologies of lignocellulose. Current Research in Green and Sustainable Chemistry 2020;3:100035.

Serna LD, Alzate CO, Alzate CC. Supercritical fluids as a green technology for the pretreatment of lignocellulosic biomass. Bioresource Technology 2016;199:113-120.

Sluiter A, Hames B, Hyman D, Payne C, Ruiz R, Scarlata C. et al. Determination of total solids in biomass and total dissolved solids in liquid process samples. National Renewable Energy Laboratory (NREL); 2008.

Stewart D. Lignin as a base material for materials applications: Chemistry, application and economics. Industrial crops and products 2008;27(2):202-207.

Yonsuwan D. Optimization of lignin concentration in black liquor using electrochemical and physical processes. Interdisciplinary Program of Environmental Science, Graduate School of Chulalongkorn University; 2010.

Zhao X, Cheng K, Liu D. Organosolv pretratment of lignocellulosic biomass for enzymatic hydrolysis. Applied Microbiology and Biotechnology 2009;82:815-827.

Zhao X, Li S, Wu R, Liu D. Organosolv fractionating pre-treatment of lignocellulose biomass for efficient enzymatic saccharification: chemistry, kinetics, and substrate structures. Biofuels, Bioproducts and Biorefining 2017;11(3):567-590




How to Cite

Yoyrurob, S., Naban, O. ., Choopia, S. ., Charnnok, B. ., & Boonsai, P. . (2021). ENHANCING THE POTENTIAL OF METHANE PRODUCTION FROM OIL PALM FIBER BY ETHANOL PRETREATMENT. Life Sciences and Environment Journal, 22(2), 308–319. https://doi.org/10.14456/lsej.2021.17



Research Articles