PRODUCTION AND ANTIOXIDANT ACTIVITY OF PROTEIN HYDROLYSATE FROM RICE SEED KERNEL

Abstract

The purposes of this research were to produce and study the antioxidant activity of protein hydrolysate from seed kernel of four rice cultivars: Ayutthaya 1, Suphanburi 1, Pathum Thani 1 and RD 29. To obtain crude protein extract, rice seed kernel was soaked in 0.2 % sodium hydroxide (NaOH) and protein content was determined according to the Lowry method. The results showed that crude protein extract from seed kernel of Pathum Thani 1 had the highest percent yield (8.01 ± 0.08 %) and the highest amount of crude protein (6.92 ± 0.02 g/100 g sample). The protein types and patterns were analyzed by SDS-PAGE technique. Electrophoretic patterns of the extracted proteins have shown a slight difference in the patterns of proteins with molecular weight between 19.3 – 102.1 kDa. The production of protein hydrolysate was prepared by hydrolysis of crude protein extract with 4 M HCl at 100°C for 6 h and antioxidant activity was analysis by DPPH method. Th results showed protein hydrolysate had higher scavenging activity than crude protein extract for all cultivars.