• Araya Bunsak Pibulsongkram Rajabhat University
  • Sudarat Sutaphan
  • Weerathep Pongprasert
  • Kanita Kertsuk




Bacillus spp., Bipolaris spp., brown spot disease, biological control


 The objective of this research was to study on the potential of Bacillus sp. isolated by soil dilution plate technique from paddy soil samples collected from Phromphiram and Thachang subdistricts, Phromphiram district, Phitsanulok Province to control brown spot disease (Bipolaris sp) in rice. The result revelled that the total of 4 Bacillus spp. isolates composed of BP01 and BP02 from Phromphiram subdistricts and BC01 and BC02 from Thachang subdistricts was found. The similar potential efficiency (p>0.05) to control Bipolaris spp of all isolates was detected at the rate of 78.69, 78.87, 75.19 and 81.81%, respectively. However, the statistical difference (p<.05) among the size of clear zone from those isolated was found. The widest clear zone was found from BC02 (3.39 cm.) followed by BP02 (2.34 cm), BP01 (2.22 CM.) and BC01 (1.64 cm.) after incubated for 14 days.


Ahmed MF, Khalequzzaman KM, Islam MN. et al. Effect of fungicides against Bipolaris oryzae of rice under in vitro condition. Pakistan Journal of Phytopathology 2002;1(1):4-7.

Bake KF, James Cook R. Biological Control of Plant Pathogen. Freeman WH, San Francisco; 1974.

Bernal G, IllanesS A, Ciampi L. Isolation and partial purification of a metabolite from a mutant strain of Bacillus sp. with antibiotic activity against plant pathogenic agents. Electronic Journal of Biotechnology 2002;5(1):12-20.

Calvo Py, Zúñiga D. Caracterización fisiológica de cepas de Bacillus spp. aisladas de la rizósfera de papa (Solanum tuberosum). Ecología Aplicada 2010:31-39.

Chiangsin R, Kesee C, Sangchote S. Biological Control of Bipolaris oryzae With Bacillus Subtilis and the Development of a Formulation for Rice Seed Treatment. Thai Journal of Agricultural Science 2018;51(3):139-151.

Czaczyk K, Stachowiak B, Trojanowska K. Antifungal activity of Bacillus sp. isolated from compost. Folia Microbiologica 2000;45(6):552-554.

Földes T, Bánhegyi I, Herpai Z. Isolation of Bacillus strains from the rhizosphere of cereals and in vitro screening for antagonism against phytopathogenic, food-born pathogenic and spoilage microorganisms. Journal of Applied Microbiology 2000;89:840-846.

Gupta V, Shamas N, Razdan VK. et al. Foliar application of fungicides for the management of brown spot disease in rice (Oryza sativa L.) caused by Bipolaris oryzae. African Journal of Agricultural Research 2013;8(25):3303-3309.

Huang TP, Der-Syh Tzeng D, Wong ACL, et al. DNA polymorphisms and biocontrol of Bacillus antagonistic to citrus bacterial canker with indication of the interference of phyllosphere biofilms. PLoS One 2012;7(7):e42124.

Intana W, Yenjit P, Suwanno T. et al. Efficacy of antifungal metabolites of Bacillus spp. for controlling tomato damping-offcaused by Pythium aphanidermatum. Walailak Journal of Science and Technology 2010;5(1):29-38.

Kabir L, Sang WK, Yun SK, et al. Application of Rhizobacteria for Plant Growth Promotion Effect and Biocontrol of Anthracnose Caused by colletotrichum acutatum on Pepper. Mycobiology 2012,40: 244-251.

Kittikanokrat W. Study of Formulation and Shelf-life of Bacillus subtilis TISTR 001 Product. Master of Science in Biotechnology, King Mongkut’s University of Technology Thonburi, 2003.

Kumar A, Saini S, Shrivastava JN. Production of peptide antifungal antibiotic and biocontrol activity of Bacillus subtilis. Indian Journal of Experimental Biology 2009;47(1):57-62.

Land Development Department. Soil Series 1:25,000 scale map, 2021. Available at: http://eis.ldd.go.th/lddeis/SoilView.aspx. Accessed April 25, 2021.

Layton C, Maldonado E, Monroy L. et al. Bacillus spp.; perspectiva de su efecto biocontrolador mediante antibiosis en cultivos afectados por fitopatógenos. Revista NOVA Publicación Científica en Ciencias Biomédicas 2011;9:177-187.

McSpadden Gardener BB. Ecology of Bacillus and Paenibacillus spp. in Agricultural Systems. Phytopathology 2004;94:1252-1258.

Meena KR, Kanwar SS. Lipopeptides as the Antifungal and Antibacterial Agents: Applications in Food Safety and Therapeutics. BioMed Research International 2015;1-9.

Muenkun S. Diversity and antagonistic potential of Bacillus sp. for Controlling of Plant Phatology. Master of Science in Plant Phatology, Khon Kaen University, 2004.

Nalk RM, Akila R, Thiruvudainambi S. Management approaches for brown spot of rice caused by Bipolaris oryzae. Journal of Farm Science 2016;29(3):370-376.

Prasanna Kumar MK, Narayanappa Amruta CP, Manjula ME, et al. Characterisation, screening and selection of Bacillus subtilis isolates for its biocontrol efficiency against major rice diseases. Biocontrol Science and Technology 2017;27(4):581-599.

Rauch, EM, Bar-Yam Y. Long-range interactions and evolutionary stability in a predator-prey system. Physical Review Journals 2006;73:e020903.

Rice Department. Plantwise Factsheets for Farmers. Plantwise Knowledge Bank, 2013. Available at: https://www.plantwise.org/FullTextPDF/2016/20167800194.pdf. Accessed November 10, 2019.

Samson R A, Evans H C, Latge J P. Atlas of Entomopathogenic Fungi. Springer-Verlag, Berlin, 1988.

Sha Y, Zeng Q, Sui S. Screening and Application of Bacillus Strains Isolated from Nonrhizospheric Rice Soil for the Biocontrol of Rice Blast. Journal of Plant Pathology 2020;36(3):231-243.

Shafi J, Tian H, Ji M. Bacillus species as versatile weapons for plant pathogens: a review. Biotechnology and Biotechnological Equipment 2017;31:446-459.

Shirokov AV, Loginov ON, Melent’ev AI, et al. Protein and peptide factors from Bacillus sp. 739 inhibit the growth of phytopathogenic fungi. Applied Biochemistry Microbiology 2002;38:139-143.

Tejera-Hernández B, Rojas-Badía MM Heydrich-Pérez YM. Potencialidades del género Bacillus en la promoción del crecimiento vegetal y el control de hongos fitopatógenos. Revista CENIC Ciencias Biológicas 2011;42:131-138.

Usuwan P. Biocontrol of Grape Fungal Diseases by Bacillus spp. and Streptomyces spp. A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Crop Production Technology Suranaree University of Technology, 2007.

Vargas-Ayala R, Rodríguez-Kábana R, Morgan-Jones G, et al. Shifts in Soil Microflora Induced by Velvetbean (Mucuna deeringiana) in Cropping Systems to Control Root-Knot Nematodes. Biological Control 2000,17:11-22.

Zuber P, Nakano MM, Marahiel MA. Peptide antibiotics. In: Sonenshein AC,Hooh JA, Losick R. eds. Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology, and molecular genetics. American Society for Microbiology: Washington DC; 1993: 897-917.




How to Cite

Bunsak, A., Sutaphan, S. ., Pongprasert, W., & Kertsuk, K. . (2021). POTENTIAL OF BACILLUS SPP. ISOLATED FROM PADDY SOIL FOR CONTROLING BROWN SPOT DISEASE, BIPOLARIS SPP., IN RICE. Life Sciences and Environment Journal, 22(2), 137–147. https://doi.org/10.14456/lsej.2021.5



Research Articles