PHYTOCHEMICAL SCREENING, DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS AND ANTIOXIDANT ACTIVITIES FROM CRATEVA MAGNA (LOUR.) DC.

  • Panitan Surayot Department of Chemistry Faculty of Science and Technology Pibulsongkram Rajabhat University Phitsanulok 65000, THAILAND 66-5526-7000#7706
  • Pattawat Seekhaw
Keywords: : Crateva magna (Lour.) DC.,, phytochemical, antioxidant activity

Abstract

This research aims to perform phytochemical screening, determine total phenolic content, flavonoid content and antioxidant properties of ethanol extracts of flowers, young leaves, mature leaves, twigs and bark of Crateva magna (Lour.) DC. The antioxidant activity was studied using DPPH, ABTS and FRAP assays. The total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were determined using Folin-Ciocalteuand and Aluminium chloride colorimetric methods, respectively. The preliminary phytochemical test results indicated the presence of phenolics, flavonoids, tannins and volatile oils in the extracts from different parts of the plant samples. The young leaves extract possessed the highest value of TPC and TFC with 5.73 ± 0.28 mg GAE/g DW and 56.62 ± 0.23 mg RUE/g DW, respectively. The young leaves extract also showed the highest antioxidant activity as evidenced by DPPH and ABTS assays at IC50 values of 0.30 ± 0.02 mg/ml and 0.92 ± 0.04 mg/ml, respectively and FRAP value of 13.06 ± 0.08 FeSO4/g DW. In conclusion, the young leaves extract of this plant showed high phenolic and flavonoid contents with strong antioxidant properties. It was also found that phenolic and flavonoid contents related to antioxidant activity. Therefore, the extracts from C. magna are good sources of antioxidants and could be developed for pharmaceutical applications.

Downloads

Download data is not yet available.

References

Abirami J, Jothi G, Brindha P. Microscopic, physicochemistry and phytochemical screening of Crateva Magna (Lour) DC. (leaf), International Journal of Current Pharmaceutical Research. 2017; 9(5): 201-204.

Adebiyi LE, Olayemi FO, Ning-Hua T. et al. In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia, Beni-Suef University Journal of Basic and Applied Sciences. 2017; 6: 10-14.

Banjarnahor SDS, Artanti N. Antioxidant properties of flavonoids, Medical Journal of Indonesia. 2014; 23(4): 239-244.

Behera S, Behera B, Barik DP. et al. Phytochemical analysis of the leaf, flower, and bark of a medicinal plant, Crataeva magna (Lour.) DC, Asian Journal of Pharmaceutical and Clinical Research. 2016; 9(1): 177-178.

Brighents IMC, Dias M, Verdi LG. et al. Antioxidant Activity and Total phenolic content of some Brazilian species, Pharmaceutical Biology. 2007; 45(2): 156-161.

Chuakul W. Indigenous medicinal plants used as paralysis remedy, Thai Pharmaceutical and Health Science Journal. 2010; 5(3): 193-201.

Embuscado EM. Spice and herbs: natural sources of antioxidants-a mini review, Journal of Functional Foods. 2015; 18: 811-819.

Kaur M, Asthir B, Mahajan, G. Variation in antioxidants, bioactive compounds and antioxidant capacity ingerminated and ungerminated grains of ten rice cultivars, Rice Science. 2017; 24(6): 349-359.

Kumar S. The importance of antioxidant and their role in pharmaceutical science-a review, Asian Journal of Research in Chemistry and Pharmaceutical Sciences. (2014); 2(1): 27-44.

Loganayaki N, Manian S. Evalution of indian sacred tree Crataeva magna (Lour.) DC. for antioxidant activity and inhibition of key enzymes relevant to hyperglycemia, Journal of Bioscience and Bioengineering. 2012; 113(3): 378-380.

Mandal S, Yadav S, Yadav S. et al. Antioxidant: a review, Journal of Chemical and Pharmaceutical Research. 2009; 1(1): 102-104.

Mekab SK, Mishra S, Sahoo S. et al. Antiurolithiatic activity of Crataeva magna Lour. bark, Indian Journal of Natural Products and Resources. 2011; 2(1): 28-33.

Neamsuvan O, Sengnon N, Seemaphrik N. et al. A survey of medicinal plants around upper songkhla lake, African Journal of Traditional, Complementary and Alternative Medicines. 2015; 12(2): 133-143.

Pang Y, Ahmed S, Xu Y. et al. Bound phenolic compounds and antioxidant properties of whole grain and bran of white, red and black rice, Food Chemistry. 2018; 240: 212-221.

Pietta PG. Flavonoids as antioxidants, Journal of Natural Products. 2000; 63: 1035-1042.

Pramai P, Jiamyangyen S. Chemometric classification of pigmented rice varieties based on ant oxidative properties in relation to color, Songklanakarin Journal of Science and Technology. 2016; 35(5): 463-472.

Radha SR, Doraiswamy R. HPTLC finger printing analysis of the aqueous bark extract of Crateva magna Lour. (DC.) for terpenoid content, World Journal of Pharmacy and Pharmaceutical Sciences. 2017; 6 (7): 1489-1495.

Radha SR, Vijayakumari B. Phytochemical investigation of the extracts of Crateva magna Lour.,JPR: BioMedRx: An International Journal. 2013); 1(8): 848-851.

Ramu RS, Doraiswamy R, Yadav HM. Antiurolithiatic sctivity of aqueous bark extract of Crat aeva magna Lour. (DC.), International Journal of Research in Ayurveda and Pharmacy. 2017; 8(2): 271-278.

Saxena M, Saxena J, Pradhan A. Flavonoids and phenolic acids as antioxidants in plants and human health, International Journal of Pharmaceutical Sciences Review and Research. 2012; 12(2): 130-134.

Shahidi P, Janitha PK, Wanasundara PD. Phenolic antioxidants. Critical Reviews in Food Science and Nutrition. 2012; 32(1): 67-103.

Tapas AR, Sakarkar DM, Kakde RB. Flavonoids as nutraceuticals: a review, Tropical Journal of Pharmaceutical Research. 2008; 7(3): 1089-1099.

Wanyo P, Schoenlechner R, Meeso N. et al. Antioxidant activities and sensory properties of rice bran with marigold tea, Food and Applied Bioscience Journal. 2014; 2(1): 1-14.

Yadav A, Kumari R, Yadav A. et al. Antioxidant and its functions in human body-a review, Research in Environment and Life Sciences. 2016; 9(11): 1328-1331.
Published
2018-09-19
How to Cite
Surayot, P., & Seekhaw, P. (2018). PHYTOCHEMICAL SCREENING, DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS AND ANTIOXIDANT ACTIVITIES FROM CRATEVA MAGNA (LOUR.) DC. Life Sciences and Environment Journal, 19(2), 297-305. Retrieved from https://ph01.tci-thaijo.org/index.php/psru/article/view/137091
Section
Research Articles