PHYTOCHEMICAL SCREENING, DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS AND ANTIOXIDANT ACTIVITIES FROM CRATEVA MAGNA (LOUR.) DC.
This research aims to perform phytochemical screening, determine total phenolic content, flavonoid content and antioxidant properties of ethanol extracts of flowers, young leaves, mature leaves, twigs and bark of Crateva magna (Lour.) DC. The antioxidant activity was studied using DPPH, ABTS and FRAP assays. The total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were determined using Folin-Ciocalteuand and Aluminium chloride colorimetric methods, respectively. The preliminary phytochemical test results indicated the presence of phenolics, flavonoids, tannins and volatile oils in the extracts from different parts of the plant samples. The young leaves extract possessed the highest value of TPC and TFC with 5.73 ± 0.28 mg GAE/g DW and 56.62 ± 0.23 mg RUE/g DW, respectively. The young leaves extract also showed the highest antioxidant activity as evidenced by DPPH and ABTS assays at IC50 values of 0.30 ± 0.02 mg/ml and 0.92 ± 0.04 mg/ml, respectively and FRAP value of 13.06 ± 0.08 FeSO4/g DW. In conclusion, the young leaves extract of this plant showed high phenolic and flavonoid contents with strong antioxidant properties. It was also found that phenolic and flavonoid contents related to antioxidant activity. Therefore, the extracts from C. magna are good sources of antioxidants and could be developed for pharmaceutical applications.
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