• Ruangwut Chutima คณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยราชภัฏพิบูลสงคราม
  • Hathaichanoke Thurian
  • Orapin Roongruang


orchid, mycorrhiza, inoculum


The aim of this research was to investigate the optimum growth conditions of orchid mycorrhizal fungi isolated from roots of 2 orchid species; Dendrobium lindleyi Steud. and Paphiopedilum callosum (Rchb.f.) Stein. The results revealed that the hyphal coils (peloton) of mycorrhizal fungi were found in the cortical root cells of these 2 orchid species. The totals of 9 fungal isolates were identified belonging to
3 genera including of Rhizoctonia, Xylaria and Fusarium. Then, the optimum temperature and pH of these fungal growth on Potato Dextrose Agar (PDA) were studied and the maximum fungal growth conditions were found after cultured at 30oC and adjusted pH of agar media to pH 5 (1 isolate) and pH 7 (8 isolates). For the study on effect of grain media and potting media on growth of fungal isolates, revealed that the suitable grain media for fungal growth was black bean and corn seed. While, the suitable of potting media was chopped coconut husk soaked for 12 hours with Potato Dextrose Broth (PDB). These results can be used for development of simple mycorrhizal inoculum production using suitable fungal growth conditions such as optimum temperature and pH for fungal cultivation on agar media, fungal mass production with suitable grain media and selection of suitable orchid potting materials for mycorrhizal inoculum production.    



Athipunyakom P, Manoch L, Piluek C. Isolation and identification of mycorrhizal fungi from eleven terrestrial orchids. Kasetsart Journal (Natural Science). 2004; 38: 216-228.

Chutima R, Dell B, Vessabutr S. et al. Endophytic fungi from Pecteilis susannae (L.) Rafin (Orchidaceae), a threatened terrestrial orchid in Thailand. Mycorrhiza. 2011; 21: 221-229.

Chutima R, Lumyong S. Production of indole-3-acetic acid by Thai native orchid-associated fungi. Symbiosis. 2012; 56: 35-44.

Dasa A, Majo'n JL, Camacho M. Effect of carbon and nitrogen sources, pH and temperature on in vitro culture of several isolates of Amanita caesarea (Scop.:Fr.) Pers. Mycorrhiza. 2006; 16: 133-136.

Douds DD, Nagahashi G, Reider C. et al. Inoculation with arbuscularmycorrhizal fungi increases the yield of potatoes in a high P soil. Biological Agriculture and Horticulture. 2007; 25: 67-78.

Kodsueb R, Lumyoung S. The first report of Aschersonia placenta Berk. on mulberry field in Thailand and effect of plant extracts to the growth of the fungus. Rajabhat Journal of Sciences, Humanities and Social Sciences. 2017; 18: 187-200.

Kumla J, Danell E, Bussaban B. et al. Suitable growth conditions and nutrition factors on in vitro culture of Phlebopus portentosus (Boletales). Chiang Mai Journal of Science. 38: 2011; 156-159.

Lee SS, Patahayah M, Chong W. et al. Successful ectomycorrhizal inoculation of twodipterocarp species with a locally isolated fungus in peninsular Malaysia. Journal of Tropical Forest Science. 2008; 20: 237-247.

Mei-ling XU, Jiao-jun ZHU, Hong-zhang K. et al. Optimum conditions for pure culture of major ectomycorrhizal fungi obtained from Pinussylvestris var. mogolica plantations in southeastern Keerqin sandy, China. Journal of Forest Research. 2008; 19: 113-118.

Robert P. Rhizoctonia-Forming Fungi a Taxonomic Guide. Great Britain: The Trustees, Royal Botanic Gardens, Kew, 1999.

Sathiyadash K, Muthukumar T, Uma E. et al. Mycorrhizal association and morphology in orchids. Journal of Plant Interactions. 2012; 7: 238-247.

Suarez PJ, Kottke I. Main fungal partners and different levels of specificity of orchid mycorrhizae in the tropical mountain forests of Ecuador. Lankesteriana. 2016; 16: 299-305.

Swarts ND, Dixon KW.Terrestrial orchid conservation in the age of extinction, Annals of Botany. 2009; 104: 543-556.

Watanabe T. Pictorial Atlas of Soil and Seed Fungi Morphologies of Cultured Fungi and Key to Species 2nded. New York: CRC Press, 2001.




How to Cite

Chutima, R., Thurian, H., & Roongruang, O. (2018). OPTIMUM GROWTH CONDITIONS OF ORCHID MYCORRHIZAL FUNGI FOR SIMPLE MYCORRHIZAL INOCULUM PRODUCTION. Life Sciences and Environment Journal, 19(1), 167–175. Retrieved from https://ph01.tci-thaijo.org/index.php/psru/article/view/103118



Research Articles