การนำไปใช้ทางชีวภาพของสาหร่ายพวงองุ่นในทางเดินอาหารจำลองของมนุษย์

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Numphon Thaiwong
Natta Kachenpukdee
Dararat Buaphet

บทคัดย่อ

การศึกษาครั้งนี้มีวัตถุประสงค์เพื่อประเมินการนำไปใช้ทางชีวภาพของสาหร่ายพวงองุ่นในแบบจำลองกระบวนการย่อยในทางเดินอาหารของมนุษย์ แบบจำลองระบบทางเดินอาหารในหลอดทดลองเป็นการทดสอบเสถียรภาพของสารประกอบฟีนอลทั้งหมดและสารต้านออกซิเดชันในสาหร่ายพวงองุ่นเมื่อผ่านกระบวนย่อย โดยศึกษาองค์ประกอบทางเคมี ปริมาณฟีนอลทั้งหมด ฤทธิ์การต้านออกซิเดชันของสาหร่ายพวงองุ่นสดและแห้ง และศึกษาการนำไปใช้ทางชีวภาพในสาหร่ายพวงองุ่นสดจากปริมาณฟีนอลทั้งหมด และฤทธิ์การต้านออกซิเดชัน ผลการทดลองพบว่า สาหร่ายพวงองุ่นสดมีปริมาณความชื้น โปรตีน ไขมัน เถ้า เท่ากับ 82.63%, 3.89%, 1.27% และ 12.21% ตามลำดับ และสาหร่ายพวงองุ่นอบแห้งมีค่าเท่ากับ 2.29%, 19.76%, 0.63% และ 31.48% ตามลำดับ ปริมาณสารประกอบฟีนอลทั้งหมดของสาหร่ายสดและสาหร่ายที่ผ่านการอบแห้ง มีค่าเท่ากับ 38.10 ± 3.00 mg GAE/g sample และ 3.50 ± 0.51 mg GAE/g sample ตามลำดับ เมื่อวิเคราะห์ฤทธิ์การต้านออกซิเดชันของสาหร่ายพวงองุ่นสดโดยวิธี DPPH และ ABTS เท่ากับ 5.90 ± 0.82% และ 13.82 ± 0.22% ตามลำดับ ขณะที่สาหร่ายพวงองุ่นแห้งมีค่าเท่ากับ 1.93 ± 0.31% และ 2.11 ± 0.13% ตามลำดับ การประเมินการนำไปใช้ทางชีวภาพของสาหร่ายพวงองุ่นสดในทางเดินอาหารจำลองของมนุษย์ แสดงให้เห็นว่า เสถียรภาพของสารประกอบฟีนอลทั้งหมดและฤทธิ์การต้านออกซิเดชันมีค่าลดลงอย่างมีนัยสำคัญทางสถิติ (p < 0.05) เมื่อปริมาณตัวอย่างเพิ่มขึ้นในระดับ 2 – 8 กรัม

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