Degradation of Penicillin G contaminant in synthesized wastewater by Fenton-like reaction

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Wiparat Nisapai
Athipong Paikamnam
Pongsert Sriprom
Sutasinee Neramittagapong
Somnuk Theerakulpisut
Chitsan Lin
Arthit Neramittagapong


The aim of this research was to investigate the degradation of Penicillin G (PEN G) in synthesized wastewater via a Fenton-like reaction. Calcium dioxide (CaO2) was used as an oxidant in the Fenton-like reaction. Factors of the PEN G degradation study such as pH, FeSO4, CaO2, and reaction time were determined using the Box-Behnken design (BBD). The experimental study was conducted in a 500 mL batch reactor, with 150 ppm initial concentration of PEN G. The results showed that PEN G degradation was optimal (38.67%) at pH 3, ferrous dosage of 0.08 g/L, CaO2 concentration of 1 g/L, and reaction time of 120 min. The degradation took place in the first 20 min. The reaction time was increased up to 120 min to achieve a pH of 8 in the test solution to meet the industrial effluent standard by the Ministry of Industry, Thailand. The kinetic study indicated second order and the pseudo first order reaction for the degradation in 0-20 min and 20-120 min, respectively. The research results indicated that pH, iron dosage, and CaO2 content-except reaction time-influenced PEN G degradation as predicted by the BBD. The degradation was substantially quick as well. Fenton-like reaction for a high concentration of PEN G should be used as pre-treatment.


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Nisapai, W., Paikamnam, A., Sriprom, P., Neramittagapong, S., Theerakulpisut, S., Lin, C., & Neramittagapong, A. (2022). Degradation of Penicillin G contaminant in synthesized wastewater by Fenton-like reaction. Engineering and Applied Science Research, 49(5), 622–629. Retrieved from


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