Chlorella sp. extraction and estimation of fuel properties of lipids derived from FFA profiles
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Abstract
Lipid extraction from marine Chlorella sp. was performed with various techniques including Soxhlet, heating and stirring with a hot plate magnetic stirrer, heating and shaking in a water bath and sonication. Cell disruption methods with sonication and microwave heating were also investigated. A single extraction in an ultrasonic bath was found to be the most suitable method. The optimum solvent system, extraction time and biomass preparation were investigated considering the extraction yield and quality parameters predicted by fatty acid compositions. Extraction in a binary solvent system, n-hexane/methanol (1:2 v/v), for 80 min using freeze-dried algae was optimal as the extraction yield was higher and the fuel properties estimated by the fatty acid composition meet biodiesel standards.
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