Efficiency of Superovulation and In Vitro Embryonic Development in ICR and BALB/c Rag2.Jak3 Double Knockout Mice
Article Sidebar
Main Article Content
Abstract
Embryo cryopreservation is a crucial method for preserving mouse strains, aiding in cost management and maintaining genetic purity. However, the efficiency of superovulation and in vitro fertilization (IVF) can vary significantly among different strains. Specific comparative data for important strains, such as the immunodeficient BALB/c Rag-2.Jak3 double knockout (BRJ) mouse strain and the control ICR strain, remain limited. Therefore, this study aimed to compare the number of retrieved oocytes, fertilization rates, and 2-cell embryo development between ICR and BRJ mouse strains. Twelve-week-old female mice (n=5/strain) were superovulated with Pregnant Mare’s Serum Gonadotropin (PMSG) and human Chorionic Gonadotropin (hCG), respectively. Retrieved oocytes were then subjected to in vitro fertilization (IVF) using sperm collected from 12-week-old male mice (n=5/strain). Embryos were subsequently cultured under controlled conditions (37°C, 5% CO2) until the 2-cell stage. Results indicated that ICR mice yielded a higher average number of retrieved oocytes (52.4 ± 29.69 oocytes/mouse) compared to BRJ mice (34.4 ± 25.18 oocytes/mouse). The fertilization and 2-cell development rates were 78.24% (205/262) for ICR and 75.00% (129/172) for BRJ. However, statistical analysis revealed no significant differences in the number of retrieved oocytes and 2-cell embryo development rates between the two strains (p > 0.05). Nevertheless, the ICR strain consistently showed a tendency towards superior oocyte yield and embryo development rates. This study indicates that the response to superovulation and embryo development in mice is strain-specific, potentially influenced by underlying physiological and genetic factors. This study reveals a variable response to hormonal stimulation between mouse strains, despite the applicability of the same protocol to both. These findings are crucial for optimizing embryo cryopreservation and provide fundamental data for establishing an embryo bank.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
ธีรพงษ์ บัวบาน, พรรัตนา ช่อมณี และนาถนภิส ประทีป ณ ถลาง (2559). การเปรียบเทียบการเจริญของตัวอ่อนระยะ 1 เซลล์ของหนูแรท สายพันธุ์ Mlac: WR ในสารละลาย M16 และ KSOM. ใน: การประชุมทาง วิชาการของมหาวิทยาลัย เกษตรศาสตร์ ครั้งที่ 54, กรุงเทพฯ. 483 - 489
Arifin, W. N. and Zahiruddin, W. M. (2017). Sample size calculation in animal studies using resourceequation approach. The Malaysian journal of medical sciences: MJMS 24(5): 101.
Brown, S.D. and Moore, M.W. (2012). The International Mouse Phenotyping Consortium: past and future perspectives on mouse phenotyping. Mammalian Genome 23: 632 - 640.
Byers, S.L., Payson, S.J. and Taft, R.A. (2006). Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs). Theriogenology 65(9): 1716 - 1726.
Byers, S.L., Wiles, M.V., Dunn, S.L. and Taft, R.A. (2012). Mouse estrous cycle identification tool and images. PloS one 7(4): e35538.
Gates, A.H. (1956). Viability and developmental capacity of eggs from immature mice treated with gonadotrophins. Nature 177: 754 - 755.
Kim, J.E., Nam, J.H., Cho, J.Y., Kim, K.S. and Hwang, D.Y. (2017). Annual tendency of research papers used ICR mice as experimental animals in biomedical research fields. Laboratory Animal Research 33: 171 - 174.
Li Shuai, L.S. and Winuthayanon, W. (2017). Oviduct: roles in fertilization and early embryo development. Journal of Endocrinology 232(1): 1 - 26.
Luo, C., Zuñiga, J., Edison, E., Palla, S., Dong, W. and Parker-Thornburg, J. (2011). Superovulation strategies for 6 commonly used mouse strains. Journal of the American Association for Laboratory Animal Science 50(4): 471 - 478.
Nakagata, N. (1993). Production of normal young following transfer of mouse embryos obtained by in vitro fertilization between cryopreserved gametes. Reproduction 99(1): 77 - 80.
Nakagata, N. (2016). Reproductive engineering techniques in mice (3rd ed.). Cosmo Bio Co., Ltd.
Nakagata, N. and Takeo, T. (2019). Basic mouse reproductive techniques developed and modified at the Center for Animal Resources and Development (CARD), Kumamoto University. Experimental Animals 68(4): 391 - 395.
Takeo, T. and Nakagata, N. (2015). Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice. PloS one 10(5): e0128330.
Takeo, T. and Nakagata, N. (2016). Immunotherapy using inhibin antiserum enhanced the efficacy of equine chorionic gonadotropin on superovulation in major inbred and outbred mice strains. Theriogenology 86(5): 1341 - 1346.
Takeo, T. and Nakagata, N. (2018). In Vitro Fertilization in Mice. Cold Spring Harbor Protocols 2018(6).
Takeo, T., Nakao, S., Nakagawa, Y., Sztein, J.M. and Nakagata, N. (2020). Cryopreservation of mouse resources. Laboratory Animal Research 36(1): 1 - 6.
Vaeteewoottacharn, K., Pairojkul, C., Kariya, R., Muisuk, K., Imtawil, K., Chamgramol, Y., Bhudhisawasdi, V., Khuntikeo, N., Pugkhem, A., and Saeseow, O.T. (2019). Establishment of highly transplantable cholangiocarcinoma cell lines from a patient-derived xenograft mouse model. Cells 8 (5): 496.
Whittingham, D.G. and Wood, M.J. (1983). Reproductive physiology. In The mouse in biomedical research San Diego: Academic Press. 137 - 164
