Cytogenetics of Dusky Langur (Trachypithecus obscurus) and Silvered Langur (T. cristatus) in Thailand by C-banding

Main Article Content

Krit Phinetsathian
Nattasuda Donbundit
Namphunk Seatung
Sumpars Khunsook

Abstract

Cytogenetics of Trachypithecus obscurus and T. cristatus from Nakhon Ratchasima Zoo were studied. Blood samples were collected and subjected to lymphocyte cultures harvested by colchicine-hypotonic-fixation-air-drying technique followed by C-banding. The results showed that T. obscurus and T. cristatus have chromosome number 2n = 44. Almost all of chromosomes of T. obscurus were stained at the centromere, and the apical of telomere including X, Y chromosomes, except 4 and 18, which were stained at the centromere. In addition, the chromosome 20 was stained at the telomere. Due to the low number of cells, we cannot draw the idiogram of the T. cristatus.

Article Details

How to Cite
Phinetsathian, K., Donbundit, N., Seatung, N., & Khunsook, S. (2024). Cytogenetics of Dusky Langur (Trachypithecus obscurus) and Silvered Langur (T. cristatus) in Thailand by C-banding. KKU Science Journal, 52(1), 47–56. https://doi.org/10.14456/kkuscij.2024.5
Section
Research Articles

References

Chaiyasut, K. (1989). Cytogenetics and Cytotaxonomy of Zephyranthes. Bangkok: Department of Botany, Faculty of Science, Chulalongkorn University.

Fukushi, D. and Ushiki, T. (2005). The structure of C-banded human metaphase chromosomes as observed by atomic force microscopy. Archives of Histology and Cytology 68(1): 81 –87. doi: 10.1679/aohc.68.81.

Hirai, H. (2001). Relationship of telomere sequence and constitutive heterochromatin in the human and apes as detected by PRINS. Chromosome Painting 23: 29 – 35. doi: 10.1007/978-94-010-0330-8_5.

Kampiranont, A. (2003). Cytogenetics, 2nd ed. Bangkok: Department of Genetics, Faculty of Science, Kasetsart University.

Kangwanpong, D. (2003). Genetics. Chiang Mai: Chiang Mai Business Center and Studio LTD.

Kanwatthanakit, C. (2000). Mammals in Thailand and Indochina. Bangkok: WWF-Thailand Project Office.

Laohachinda, N. (1996). Evolution of animals. Department of Genetics, Faculty of Science, Kasetsart University, Bangkok.

Lekagul, B. and McNeely, J.A. (1977). Mammals of Thailand. 1st ed. Bangkok: Association for the conservation of wildlife, Kurusapha Ladprao Press.

Lertcharnrit, T. (2007). Tracing the origin of man. Bangkok: Sarakadee Press.

Myers, R.H. and Shafer, D.A. (1979). Hybrid Ape Offspring of a Mating of Gibbon and Siamang. Science, 205(4403): 308 – 310. doi: 10.1126/science.451603.

Parr, J.W.K. (2003). A guide to the large mammals of Thailand. Bangkok: Sarakadee Press.

Pinthong, K., Tanomtong, A., Khunsook, S., Patawang, I., Wonkaonoi, W. and Supanuam, P. (2018). Karyological analysis and NOR polymorphism of phayre’s langur, Trachypithecus phayrei crepuscula (Primate, Colobinae) in Thailand. The Nucleus 61: 61 – 67.

Rooney, D.E. (Ed.). (2001). Human cytogenetics: constitutional analysis: a practical approach (Vol. 1). Practical Approach. Oxford: University Press.

Tanomtong, A., Khunsook, S., Wonkaonoi, W., Supanuam, P., Srisamoot, N. and Jumrusthanasan, S. (2014). The first karyological study and natural NOR polymorphism in Banded langur, Presbytis femoralis (Primate, Colobinae). Cytologia 79(1): 29 – 39.

van Tuinen, P. and Ledbetter, D.H. (1983). Cytogenetic comparison and phylogeny of three species of hylobatidae. American Journal of Physical Anthropology 61(4): 453 – 466. doi: 10.1002/ajpa.1330610408.

Wijayanto, H., Hirai, Y., Kamanaka, Y., Katho, A., Sajuthi, D. and Hirai, H. (2005). Patterns of C-heterochromatin and telomeric DNA in two representative groups of small apes, the genera Hylobates and Symphalangus. Chromosome Research 13(7): 715 – 722. doi: 10.1007/s10577-005-1007-4.

Wilson, D.E. and Cole, F.R. (2000). Common Names of Mammals of the World. Washington: Smithsonian Institution Press.