Antioxidant activity, Antibacterial and Anti-biofilm formation of Curcuma zedoaria extract against Streptococcus mutans
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Abstract
Streptococcus mutans is a pathogenic oral bacterium that is the main cause of tooth decay. The objective of this research was evaluated the effect of Curcuma zedoaria extract on anti-oxidation, bacteria inhibition and resist the formation of biofilms. The quantitative study of total phenolic compounds and total flavonoid showed that Curcuma zedoaria contained total phenolic compounds and flavonoids. The antioxidant activity of DPPH, ABTS and FRAP assay was then studied. The result of antioxidant activity shown with IC50 values of 58.86 ± 1.98, 22.69 ± 3.91 µg/ml and 230.76 ± 0.25 mg FeSO4 equivalent/g extracts. Antibacterial activity by Disc diffusion method showed inhibition zone were 10.46 ± 0.55 mm at concentration 500 mg/mL. It was tested for antibacterial, bactericidal activity and resist biofilm formation. The results showed that the activity of Curcuma zedoaria extract had MIC and MBC values of 100 and 120 mg/ml and the extracts can inhibit biofilm formation. This was consistent with testing the effect of Curcuma zedoaria extract on bacterial biofilm formation under a phase contrast microscope. When the extract concentration increased, the amount of biofilm formation decreased. This research shows that Curcuma zedoaria extract is effective for further development as an oral antimicrobial product.
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