Karyological characteristics of Amphiprion frenatus and Premnas biaculeatus (Perciformes, Amphiprioninae)

Abstract

The karyotypic characteristics of tomato anemonefish, Amphiprion frenatus and spine cheek anemonefish, Premnas biaculeatus from Andaman sea, Southern of Thailand were obtained from the present study. The mitotic chromosomes were prepared by direct method from kidney tissues after in vivo colchicine treatment. Conventional staining technique was used to stain the metaphase chromosome with Giemsa’s solution. The results showed that the diploid chromosome number (2n) of A. frenatus and P. biaculeatus were 48, with the fundamental numbers (NF) being 94 in both males and females. The chromosomes of A. frenatus and P. biaculeatus present metacentric, submetacentric, acrocentric, and telocentric chromosomes as 16-20-10-2 and 14-26-6-2, respectively. No morphologically differentiated sex chromosomes were detected in both sexes. The karyotype formulae were as follows:

     A. frenatus; 2n (48) = L^m₁₀+L^sm₁₄+ L^a₁₀+L^t₂+M^m₆+M^sm₆

                       2n (48) = 16m+20sm+10a+2t

    P. biaculeatus; 2n (48) = L^m₆+L^sm₁₀+ L^a₄+M^m₈+M^sm₁₆+M^a₂ M^t₂

                          2n (48) = 14m+26sm+6a+2t