The Culture of Cordyceps militaris (L.) Link with Raw Egg and Hard-Boiled Egg from Poultry

Abstract

The culture of Cordyceps militaris (L.) Link on 70 ml per bottle of the egg with six culture media formulas (the raw hen eggs, hard-boiled hen eggs, raw hen quail eggs, hard-boiled quail eggs, raw duck eggs and hard-boiled ducked eggs) were mixed with 4.00 ml of supplementary foods per bottle (KH₂PO₄ 1.00, glucose 15.00, MgSO₄ 0.50, peptone 10.00, thiamine 0.50 and yeast Extract 10.00 g per 1,000 ml of the distillated water), then cultivated the cultures for 60 days. The results found the mycelia were growth in every culture media formulas, especially the hard-boiled ducked egg formula found the fastest time for the mycelia fully covered on the culture medium with 4.80 days. The mycelia were change the color from white to yellow-orange and found the exudates, the mycelia were dense in 30 days and the time for the mycelia were develop to the primordium in 22.60 days. The develop time from the primordium to the stroma was 26.40 days. At 60 days, the storma were found orange color with clavate shape with the average 39.20, the average length of 4.62 cm, the average fresh weight of 18.14 g and cordycepin 2,857.11 mg/100 g dry weight.