DNA markers screening for developing aromatic glutinous rice lines resistance to blast and tolerance to submergence

Abstract

Developing rice varieties for multi-stress tolerance traits can be accomplished by using marker-assisted selection (MAS). Those DNA markers underlying the target traits have to distinguish polymorphism between parental lines. This research was aimed to screen DNA markers which can distinguish polymorphism between the parental lines in order to use as a tool to select favorable alleles for developing aromatic glutinous rice lines resistance to blast and tolerance to submergence. The breeding work was performed using pedigree method. Jasmine IR57514, the aromatic Jasmine-liked cooking quality and tolerance to submergence rice line, was used as female parent whereas RD6-blast No.334, a glutinous rice line resistance to blast, was used as male parent. DNA markers underlying 5 target traits were used to select progenies carrying the favorable alleles of those traits of interest in each generation which consisted of submergence tolerance (Sub1), grain aroma (badh2) and low gelatinization temperature (SSIIa-TT) from female parent and blast resistance (qBl1 and qBl11) and glutinous (wx) traits from male parent. Parental polymorphism screening was carried out using 16 DNA markers. The results showed that 8 DNA markers clearly distinguished the parental lines which consisted of 4 genes specific markers including R10783 indel, Aromarker Wx-Glu-23,and SNP2340-41 underlying submergence tolerance, grain aroma, glutinous and low gelatinization temperature traits, respectively and 4 rice microsatellite makers, RM212/RM319 and RM144/RM1233, flanking qBl1 and qBl11 conferring blast resistance traits on chromosome 1 and 11 respectively. The chosen DNA markers were employed to select good plant type and agronomic characters of 105 F₂ progenies and 9 F₂ progenies were identified carrying the favorable alleles underlying those target traits. The selected F₂ progenies were self-pollinated to produce F₃ progenies. Then, marker-assisted selection will be performed to select the F₃ progenies carrying the homozygous alleles of those traits of interest to develop aromatic glutinous rice lines resistance to blast and tolerance to submergence.