Comparisons of Sericin Protein Extraction Methods from Thai silk and their Protein Patterns

Abstract

Water soluble sericin protein from waste silk thread of 3 subspecies of Bombyx mori L.; Nang-Tui, Nang-Noi Sri-Sa-Ket and Leung-Pairoj were extracted at 25 and 100 ^oC for 0 - 60 min. Yield, protein quantity and SDS-PAGE protein pattern were analyzed. Extraction at 25 ^oC for 60 min did not give neither protein yield not protein pattern. However, extraction at 100 ^oC for 30 and 60 min gave greater yield and protein than extraction for 0 min. Higher yield and protein were given from Nang-Noi Sri-Sa-Ket and Leung-Pairoj more than Nang-Tui. SDS-PAGE protein patterns of Nang-Tui, Nang-Noi Sri-Sa-Ket and Leung-Pairoj showed protein bands in range of 100 and 250 kDa.