Tissue Culture and gus Gene Transformation into Young Leaf of Angelonia goyazensis Benth.

Abstract

The objective of this research was to study tissue culture and gus gene transformation into A. goyazensis. In vitro culture of young leaf of A. goyazensis using MS medium supplemented with 0-0.5 mg/L of TDZ and IAA was studied. Highest shoots regeneration, 38.21 shoots per explants was found on MS medium supplemented with 0.3 mg/L TDZ and 0.1 mg/L IAA. The MS medium supplemented with 0.1 mg/L IAA induced rooting of all shoots with the highest average number of 5.3 roots per shoot. A study on the effect of antibiotics on growth of young leaf of A. goyazensis was conducted for 6 weeks. The highest concentration of cefotaxime in the medium that A. goyazensis young leaf can grow and regenerate shoots without significant difference from the control was 250 mg/L, while 25 mg/L kanamycin was sufficient for inhibition of young leaf growth. The gus gene transformation into young leaf of A. goyazensis by using Agrobacterium tumefaciens strain LBA4404 (pSTART) for 20 minutes resulted in the highest transformation efficiency of 32.30 percent and gus gene expression was confirmed by gus assay