Isolation of the cellulase enzyme-producing fungi for utilizing in biofertilization

Abstract

The purpose of this study to isolate the cellulase enzyme-producing fungi from soil samples collected from different areas in Uttaradit Province. A total of 15 cellulase enzyme-producing isolates were found on Carboxy methyl cellulose agar. Among these isolates, three of them including isolates B, D and E produced the highest number of cellulase enzyme (HC value) at 2.11, 1.88 and 1.79, respectively. These three isolates were then applied in six formulas of biofertilization and used for 20 days to test for the agricultural waste degradation efficiency. At the end, all of formulas had pH ranging 7.9 - 8.5. Total nitrogen (TKN) was varied between 1.01 and 1.14%. Organic matter (OM) number was varied ranging from 18.83 - 33.62%. The carbon-nitrogen ratio (C/N ratio) had ranged between 10.26 and 17.30. Among six fertilizer formulas, formula-6 (isolates B, D and E) is the best one in terms of degradation efficiency followed by formula-3 (isolate B). Isolate B was identified as Aspergillus allahaba.