Effects of Dietary Probiotic Supplementation on Growth Enhancement and Immune Against Aeromonas in Pangasius bocourti

Abstract

The work aimed to study of dietary effective microorganism (EM) supplementation on the growth and immune enhancement for prevention of Aeromonas in Pangasius bocourti culture was divided into 24 groups at density 10, 20, 30 and 40 fishes/100 L and EM ratio 0, 100, 200, 300, 400 and 500 ml/1 kg of feed for 8 weeks. The results found that there was not effect from EM ratio on length gain, length gain per fish, length gain per fish per day, weight gain, weight gain per fish, average daily weight gain (ADG) food conversion ratio (FCR) and survival rate (p>0.05). For density, the culture at density 20 and 30 fishes/100 L had significantly higher weight gain, weight gain per fish and average daily weight gain (ADG) than density 10 and 40 fishes/100 L (p<0.05). However, there was no statically significant data of survival rate between density (p>0.05). The results of immune analysis using ELISA against Aeromonas hydrophila showed that Pangasius bocourti did not produce specific antibody against Aeromonas hydrophila. The results of disc diffusion exhibited that there was no antimicrobial activity against Aeromonas hydrophila. From the results concluded that there was no effect of dietary effective microorganism (EM) supplementation on the growth and immune enhancement for prevention of Aeromonas in good feed and water quality management of Pangasius bocourti culture.