Phytochemical Screening and Antioxidant Activity of Clerodendrum disparifolium Leaves

Abstract

Phytochemical screening of the ethyl acetate and 95% ethanolic extracts from the dried leaves of C. disparifolium revealed the presence of bioactive substances such as flavonoids and alkaloids. The anthraquinones, terpenoids, tannins, saponins and cardiac glycosides were also tested but not found. The antioxidant activity and total phenolic content were evaluated according to DPPH assay and Folin-Ciocalteu method, respectively. The results showed that ethyl acetate and ethanolic extracts had antioxidant activities calculated in IC₅₀, 4.46±0.04, 4.03±0.14 mg/mL, respectively. The total phenolics content of the ethyl acetate extract was
258.84±3.84 mg GAE/g extract whereas the ethanolic extract was 289.49±1.32 mg GAE/g extract. The correlation between antioxidant activity and total phenolics content was observed with correlation coefficient 0.8191.