Screening of Pyrethroid Resistance Bacteria from Agricultural Soil
Keywords:
Pyrethroid, Bioremediation, BiodegradationAbstract
The objective of this study is to screen and study preliminary characteristics of pyrethroid resistant bacteria from agricultural soil. Bacteria were isolated from pyrethroid contaminated soil using enrichment culture technique with serial dilution and culture on Tryptic Soy Agar (TSA). The results showed that 3 isolates of bacteria were screened. The isolates were characterized by staining and different biochemical tests. The results indicated that the isolate PT1 and PT3 would probably be Staphylococcus sp. and the isolate PT2 should be Bacillus sp. The pyrethroid minimum inhibitory concentration (MIC) study found that the highest MIC was 7.5 mM which was the MIC of isolate PT1.
Downloads
Download data is not yet available.
References
พันธุ์เครือ ทิพย์โสด, ฐปน ชื่นบาล, ศิราภรณ์ ชื่นบาล และณิชมน ธรรมรักษ์ (2558). การศึกษาประสิทธิภาพของแบคทีเรียในการย่อยสลายสารไดโคโฟล. การประชุมแห่งชาติมหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตกำแพงแสน ครั้งที่ 9 (น.1158-1165). มหาวิทยาลัยเกษตรศาสตร์ กำแพงแสน. ไทย. สืบค้นจาก https://esd.kps.ku.ac.th/kuk-conference/img/gallery/article_9/pdf/o_plant09.pdf
ศิริพรรรณ สารินทร์. (2550). จุลชีววิทยาสิ่งแวดล้อม. พิษณุโลก: สำนักพิมพ์มหาวิทยาลัยนเรศวร
สถาบันอาหาร กระทรวงอุตสาหกรรม. (ม.ป.ป). สารกำจัดแมลงและศัตรูพืช กลุ่มไพรีทรับและสารสังเคราะห์ไพรีทรอยด์ (Pyrethrum and Pyrethroides). สืบค้น 26 มกราคม 2564, จาก http://fic.nfi.or.th/foodsafety/upload/damage/pdf/pyrethrum_and_pyrethroides_2.pdf
สำนักงานคณะกรรมการอาหารและยา. (2552). ปลอดยุงปลอดภัยถ้าถ้าใช้ยากันยุงถูกวิธี. สืบค้น 25มกราคม 2564, จาก https://db.oryor.com/databank
Antwi, F., and Reddy, G.V.P. (2015). Toxicological effects of pyrethroids on non-target aquatic insects. Toxicol. Pharmacol., 40, 915-923. doi: 10.1016/j.etap.2015.09.023
Das, R., Das, S. J., and Das, A. C. (2016). Effect of synthetic pyrethroid insecticides on N2-fixation and its mineralization in tea soil. Eur. J. Soil Biol. 74, 9–15. doi: 10.1016/j.ejsobi.2016.02.005
Grant, R., and Betts, W. (2004). Mineral and carbon usage of two synthetic pyrethroid degrading bacterial isolates. Journal of Applied Microbiology. 97(3), 656-662. doi: 10.1111/j.1365-2672.2004.02358.x
Ifediegwu, MC. (2015). Isolation, Growth and Identification of Chlorpyrifos degrading bacteria from agricultural soil in Anambra State, Nigeria. Universal Journal of Microbiology Research. 3(4), 46-52. doi: 10.13189/ujmr.2015.030402
Jabeen, F., Ahmed, M., Ahmed, F., Biala, Sawar, B.M., Akhtar, S., and Shahid, A.A. (2017). Characterization of cypermethrin degrading bacteria: A hidden micro flora for biogeochemical cycling of xenobiotics. Advancements in life sciences. 4(3), 97-107. http://www.als-journal.com/articles/vol4issue3/435.17/241.pdf
Jilani, S., and Altaf Khan, M. (2004). Isolation, characterization and growth response of pesticides degrading bacteria. Journal of Biological Sciences. 4(1), 15-20. doi: 10.3923/ibs.2004.15.20
Mariusz, C. and Zofia, P-S. (2016). Pyrethroid-degrading microorganisms and their potential for their bioremediation of contaminated soils: A review. Frontier in Microbiology. 7, 1-26. doi: 10.3389/fmicb.2016.01463
Naphade, S.R., Durve, A.A., Bhot, M., Varghese, J. and Chandra, N. (2012). Isolation, characterization and identification of pesticide tolerating bacteria from garden soil. European Journal of Experimental Biology. 2(5), 1943-1951. https://www.imedpub.com/articles/isolation-characterization-and-identification-of-pesticide-tolerating-bacteria-from-garden-soil.pdf
Pandey, P., Pant, G., and Sibi, G. (2014). Isolation and characterization of bifenthrin catabolizing bacterial strain Bacillus cibi from soil for pyrethroids biodegradation. Online Journal of Biological Sciences. 14(3), 188-195. doi: 10.3844/ojbsci.2014.188.195
Tallur P.N., Megadi V.B., and Ninneka H.Z. (2008). Biodegradation of cypermethrin by Micrococcus sp. Strain CPN 1. Biodegradation. 19, 77-82. doi: 10.1007/s10532-007-9116-8
Tejada, M., García, C., Hernández, T., and Gómez, I. (2015). Response of soil microbial activity and biodiversity in soils polluted with different concentrations of cypermethrin insecticide. Arch. Environ. Contam. Toxicol. 69, 8–19. doi: 10.1007/s00244-014-0124-5
Vijverberg, HP., Vanden B.J. (1990). Neurotoxicological effects and the mode of action of pyrethroid insecticides. Critical Reviews in Toxicology. 21(20), 105-126 http://www.als-journal.com/articles/vol4issue3/435.17/241.pdf
Xu, Z., Shen, X., Zhang. X.-C., Liu, W., and Yang, F. (2015). Microbial degradation of alpha-cypermethrin in soil by compound-specific stable isotope analysis. Journal of Hazardous Mater. 295, 37-42. doi: 10.1016/j.jhazmat.2015.03.062
ศิริพรรรณ สารินทร์. (2550). จุลชีววิทยาสิ่งแวดล้อม. พิษณุโลก: สำนักพิมพ์มหาวิทยาลัยนเรศวร
สถาบันอาหาร กระทรวงอุตสาหกรรม. (ม.ป.ป). สารกำจัดแมลงและศัตรูพืช กลุ่มไพรีทรับและสารสังเคราะห์ไพรีทรอยด์ (Pyrethrum and Pyrethroides). สืบค้น 26 มกราคม 2564, จาก http://fic.nfi.or.th/foodsafety/upload/damage/pdf/pyrethrum_and_pyrethroides_2.pdf
สำนักงานคณะกรรมการอาหารและยา. (2552). ปลอดยุงปลอดภัยถ้าถ้าใช้ยากันยุงถูกวิธี. สืบค้น 25มกราคม 2564, จาก https://db.oryor.com/databank
Antwi, F., and Reddy, G.V.P. (2015). Toxicological effects of pyrethroids on non-target aquatic insects. Toxicol. Pharmacol., 40, 915-923. doi: 10.1016/j.etap.2015.09.023
Das, R., Das, S. J., and Das, A. C. (2016). Effect of synthetic pyrethroid insecticides on N2-fixation and its mineralization in tea soil. Eur. J. Soil Biol. 74, 9–15. doi: 10.1016/j.ejsobi.2016.02.005
Grant, R., and Betts, W. (2004). Mineral and carbon usage of two synthetic pyrethroid degrading bacterial isolates. Journal of Applied Microbiology. 97(3), 656-662. doi: 10.1111/j.1365-2672.2004.02358.x
Ifediegwu, MC. (2015). Isolation, Growth and Identification of Chlorpyrifos degrading bacteria from agricultural soil in Anambra State, Nigeria. Universal Journal of Microbiology Research. 3(4), 46-52. doi: 10.13189/ujmr.2015.030402
Jabeen, F., Ahmed, M., Ahmed, F., Biala, Sawar, B.M., Akhtar, S., and Shahid, A.A. (2017). Characterization of cypermethrin degrading bacteria: A hidden micro flora for biogeochemical cycling of xenobiotics. Advancements in life sciences. 4(3), 97-107. http://www.als-journal.com/articles/vol4issue3/435.17/241.pdf
Jilani, S., and Altaf Khan, M. (2004). Isolation, characterization and growth response of pesticides degrading bacteria. Journal of Biological Sciences. 4(1), 15-20. doi: 10.3923/ibs.2004.15.20
Mariusz, C. and Zofia, P-S. (2016). Pyrethroid-degrading microorganisms and their potential for their bioremediation of contaminated soils: A review. Frontier in Microbiology. 7, 1-26. doi: 10.3389/fmicb.2016.01463
Naphade, S.R., Durve, A.A., Bhot, M., Varghese, J. and Chandra, N. (2012). Isolation, characterization and identification of pesticide tolerating bacteria from garden soil. European Journal of Experimental Biology. 2(5), 1943-1951. https://www.imedpub.com/articles/isolation-characterization-and-identification-of-pesticide-tolerating-bacteria-from-garden-soil.pdf
Pandey, P., Pant, G., and Sibi, G. (2014). Isolation and characterization of bifenthrin catabolizing bacterial strain Bacillus cibi from soil for pyrethroids biodegradation. Online Journal of Biological Sciences. 14(3), 188-195. doi: 10.3844/ojbsci.2014.188.195
Tallur P.N., Megadi V.B., and Ninneka H.Z. (2008). Biodegradation of cypermethrin by Micrococcus sp. Strain CPN 1. Biodegradation. 19, 77-82. doi: 10.1007/s10532-007-9116-8
Tejada, M., García, C., Hernández, T., and Gómez, I. (2015). Response of soil microbial activity and biodiversity in soils polluted with different concentrations of cypermethrin insecticide. Arch. Environ. Contam. Toxicol. 69, 8–19. doi: 10.1007/s00244-014-0124-5
Vijverberg, HP., Vanden B.J. (1990). Neurotoxicological effects and the mode of action of pyrethroid insecticides. Critical Reviews in Toxicology. 21(20), 105-126 http://www.als-journal.com/articles/vol4issue3/435.17/241.pdf
Xu, Z., Shen, X., Zhang. X.-C., Liu, W., and Yang, F. (2015). Microbial degradation of alpha-cypermethrin in soil by compound-specific stable isotope analysis. Journal of Hazardous Mater. 295, 37-42. doi: 10.1016/j.jhazmat.2015.03.062
Downloads
Published
2021-03-31
Issue
Section
Research Article
