การสังเคราะห์และฤทธิ์ต้านอนุมูลอิสระของโพรบฟลูออเรสเซนต์ฟลาโวนอล (3-hydroxy-2-(pyren-1-yl)-4H-chromen-4-one)
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งานวิจัยนี้มีจุดประสงค์เพื่อศึกษาสมบัติทางแสง และฤทธิ์ในการต้านอนุมูลอิสระด้วยวิธี DPPH Assay ปริมาณฟีนอลิกรวม และปริมาณฟลาโวนอยด์รวมของโพรบฟลูออเรสเซนต์ฟลาโวนอล (3-hydroxy-2-(pyren-1-yl)-4H-chromen-4-one) โดยฟลาโวนอลที่ได้นำมาทดสอบสมบัติการดูดกลืนแสงและการเปล่งแสง ทดสอบฤทธิ์ต้านอนุมูลอิสระด้วย 2,2-diphenyl-1-picrylhydrazyl (DPPH) ทดสอบปริมาณฟีนอลิกรวมด้วย Folin-Ciocalteau’s Reagent โดยใช้กรดแกลลิกเป็นสารมาตรฐาน และทดสอบปริมาณฟลาโวนอยด์รวมโดยใช้เควอซิทินเป็นสารมาตรฐาน ผลการศึกษาพบว่าฟลาโวนอลมีสมบัติการดูดกลืนแสงโดยกระตุ้นแสงที่ 355 นาโนเมตร และค่าการเปล่งแสงสูงสุดที่ความยาวคลื่น 418 นาโนเมตร (Ex 355 nm/Em 418 nm) นอกจากนี้ยังพบว่าสารสังเคราะห์ฟลาโวนอลมีฤทธิ์ต้านอนุมูลอิสระ โดยมีเปอร์เซ็นต์การต้านออกซิเดชันของ DPPH Radicals อยู่ที่ 44.7 เปอร์เซ็นต์ ที่ 10 mg/mL มีปริมาณฟีนอลิกรวมและปริมาณฟลาโวนอยด์รวมเท่ากับ 1.239±0.043 mgGAE/gDW และ 1.312±0.014 mgCE/gDW จะเห็นว่าสารสังเคราะห์ฟลาโวนอลมีคุณสมบัติตานออกซิเดชันที่ดี ซึ่งสามารถนำข้อมูลจากการวิจัยนี้ไปพัฒนาเป็นผลิตภัณฑ์และพัฒนาในทางการแพทย์ต่อไป
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