Electrochemical Determination of Vitamin C Using Antimony Film Modified Pencil Electrode
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Abstract
Vitamin C, or ascorbic acid, is an important nutrient necessary for maintaining human health. This proposed study aims to develop a simple and sensitive electrochemical sensor for quantifying ascorbic acid. The antimony film (Sb) with Nafion (NF) was prepared by ex-situ method on a supporting pencil lead electrode (Pencil Lead Electrode, PLE). Ascorbic acid exhibits a very sensitive anodic peak at +0.4 V (vs. Ag/AgCl) in a pH 4 phosphate buffer solution when using cyclic voltammetry with the PLE/NF/Sb. Amperometry, accurate and fast technique, was utilized for quantitative analysis, showing a good linear relationship between current and ascorbic acid concentration within a linear range of 50 - 500 µM (R2 = 0.9959) with limit of detection, LOD (3SD/m) 2.57 µM. Several potentially interfering species, including ionic and organic compounds, did not have any significant effect on the determination of ascorbic acid. The final experimental results revealed that the proposed modified electrochemical sensor successfully analyzed commercial pharmaceutical ascorbic acid tablets.
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