The Influence of Primed Seed with KNO3, KH2PO4 and GA3 on Germination and Seedling Growth of Water Morning Glory (Ipomoea aquatica Forsk. Var. reptan)

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Jakkrapong Kangsopa
Phetcarat Jeephet
Chuthamat Atnaseo

Abstract

Water morning glory is one of the popular vegetables used in cooking among Thai people, such that there are widely established water morning glory seed business. However, industrial scale production of water morning glory is faced with problems of easily deteriorating seeds resulting in slowed and uneven germination and abnormal seedling. Therefore, the objective of this experiment was to find suitable ratio of KNO3, KH2PO4 and GA3 for the priming of morning glory seeds and to observe changing in germination rate, seedling vigor and growth parameter of morning glory seedlings. Study was conducted at the seed technology laboratory, Faculty of Agricultural Production, Maejo University. Eleven different treatments were separately applied to morning glory seeds, which included no treatment, and treating by priming with distilled water, with 1, 2 or 3 % of KNO3 (% W/V), with 1, 2 or 3 % of KH2PO4, and with 0.02, 0.05 or 1 % of GA3 (% W/V). It was found that water morning glory seeds primed with 2 % KH2PO4 displayed the increases in radicle emergence, speed of radicle emergence, germination percentage, speed of germination and germination index when compared to untreated seeds. Priming with 1 % GA3 resulted in higher germination rate of primed seeds compared to unprimed seeds. Moreover, priming with 1 % GA3 also resulted in increasing seedling shoot length and total seedling length that were different, statistically, when compared primed to unprimed seeds.

Article Details

How to Cite
[1]
J. Kangsopa, P. Jeephet, and C. Atnaseo, “The Influence of Primed Seed with KNO3, KH2PO4 and GA3 on Germination and Seedling Growth of Water Morning Glory (Ipomoea aquatica Forsk. Var. reptan)”, RMUTI Journal, vol. 14, no. 2, pp. 28–44, Jun. 2021.
Section
Research article

References

Sarepoua, E., Khaengkhan, P., and Aekaraj, C. (2018). Effects of Varieties and Seedling Medias on Growth and Yields in Water Convolvulus Sprouts Production. Khon Kaen Agriculture Journal. Vol. 46, pp. 543-548

Phokawattana, C. and Chavapradit, P. (1996). Water Morning Glory. Bangkok: Department of Agricultural Extension

The Office of Agricultural Regulation. (2019). Quantity and Value of Export of Controlled Seeds. Access (10 December 2020). Available (https://bit.ly/39VenkH)

Charoensuk, S. (1996). Handbook of Vegetable Garden. Bangkok: Petchkarat Printing

Maneerat, C., Rithichai, P., and Jirakiattikul, Y. (2013). Effects of Salicylic Acid and Folic Acid Priming on Germination, Vigor and Seedling Growth of Kangkong. Thai Journal of Science and Technology. Vol. 21, Supplement 6, pp. 511-519

Taylor, A. G., Allen, P. S., Bennett, M. A., Bradford, K. J., Burris, J. S., and Misra, M. K. (1998). Seed Enhancements. Seed Science Research. Vol. 8, Issue 2, pp. 245-256

McDonald, M. B. (2000). Seed Priming. pp. 287-325, In Black, M. and Bewley, J. D. (Eds.), Seed Technology and Its Biological Basis, Sheffield Academic Press, England

Siri, B. (2015). Seed Conditioning and Seed Enhancements. Khon Kaen: Klungnanawitthaya

Printing

Osuna, D., Prieto, P., and Aguilar, M. (2015). Control of Seed Germination and Plant Development by Carbon and Nitrogen Availability. Frontiers in Plant Science. Vol. 6, p. 1023. DOI: 10.3389/fpls.2015.01023

Yang, W. (2018). Effect of Nitrogen, Phosphorus and Potassium Fertilizer on Growth and Seed Germination of Capsella Bursa-Pastoris (L.) Medikus. Journal of Plant Nutrition. Vol. 41, Issue 5, pp. 636-644. DOI: 10.1080/01904167.2017.1415350

Sivanesan, I., Son, M. S., Lim, C. S., and Jeong, B. R. (2011). Effect of Soaking of Seeds in Potassium Silicate and Uniconazole on Germination and Seedling Growth of Tomato Cultivars, Seogeon and Seokwang. African Journal of Biotechnology. Vol. 10, No. 35, pp. 6743-6749

Qing, Y. W. (2006). Effects of GA3, 6-BA and 2, 4-D Applied in Cucumber Seed Film Coating (Abstract). Access (May 1, 2017). Available (https://goo.gl/UdnoN6)

Siri, B., Klarod, K., and Harnsuri, J. (2016). Effects of Seed Coating with Plant Hormones on Hybrid Tomato Seeds. Khon Kaen Agriculture Journal. Vol. 44, Supplement 1, pp. 345-349

ISTA (International Seed Testing Association). (2019). International Rules for Seed Testing, Edition 2019. Bassersdorf: International Seed Testing Association

Czabator, F. J. (1962). Germination Value: An Index Combining Speed and Completeness of Pine Seed Germination. Forest Science. Vol. 8, pp. 386-395. DOI: 10.1093/FORESTSCIENCE/8.4.386

Abdul-Baki, A. A. and Anderson, J. D. (1973). Vigor Determination in Soybean Seed by Multiple Criteria. Crop Science. Vol. 13, Issue 6, pp. 630-633. DOI: 10.2135/cropsci1973.0011183X001300060013x

Ellis, R. A. and Roberts, E. H. (1981). The Quantification of Ageing and Survival in Orthodox Seeds. Seed Science and Technology. Vol. 9, Issue 2, pp. 373-409

Hilton, T. R. and Thomas, J. A. (1986). Regulation of Pregerminative Rates of Respiration in Seeds of Various Seed Species by Potassium Nitrate. Journal of Experimental Botany. Vol. 37, No. 183, pp. 1516-1524

Theerakulpisut, P. (1997). Plant Physiology. Khon Kaen: Department of Biology, Faculty of Science, Khon Kaen University

Bunnag, S. (1999). Plant Physiology. Khon Kaen: Department of Biology, Faculty of Science, Khon Kaen University

Marschner, H. (1995). Miniral Nutrition of Higher Plant. 2nd Edition. Stuttgart: Institute of Plant Nutrition, University of Ohenheim, Germany

Iglesias, R. G. and Babiano, M. J. (1997). Endogenous Abscisic Acid During the Germination of Chick-Pea Seed. Physiologia Plantarum. Vol. 100, Issue 3, pp. 500-504. DOI: 10.1111/j.1399-3054.1997.tb03054.x

Liu, P. P., Koizuka, N., Homrichhausen, T. M., Hewitt, J. R., Martin, R. C., and Nonogaki, H. (2005). Large-Scale Screening of Arabidopsis Enhancer-Trap Lines for Seed Germination-Associated Genes. The Plant Journal. Vol. 41, Issue 6, pp. 936-944. DOI: 10.1111/j.1365-313X.2005.02347.x

Hentrich, M., Boettcher, C., and Duchting, P. (2013). The Jasmonic Acid Signaling Pathway is Linked to Auxin Homeostasis Through the Modulation of YUCCA8 and YUCCA9 Gene Expression. The Plant Journal. Vol. 74, Issue 4, pp. 626-637. DOI: 10.1111/tpj.12152

Reza, D., Hassandokht, M. R., and Nazeri, V. (2015). Effects of Moist Stratification, GA3 and Seed Age on Seed Germination of Rheum Khorasanicum B. Baradaran & A. Jafari. Journal of Applied Research on Medicinal and Aromatic Plants. Vol. 2, Issue 4, pp. 168-173. DOI: 10.1016/j.jarmap.2015.07.001

Turkyilmaz, B. (2012). Effects of Salicylic and Gibberellic Acids on Wheat (Triticum aestivum I.) Under Salinity Stress. Bangladesh Journal of Botany. Vol. 41, No. 1, pp. 29-34. DOI: 10.3329/bjb.v41i1.11079

Chanprasert, W. (2010). Seed Physiology. Bangkok: Faculty of Agriculture Kasetsart University

Taiz, L. and Zeiger, E. (2010). Plant Physiology. 5th Edition. Massachusetts: Sinauer Associates

Davies, P. J. (2010). Plant Hormones: Biosynthesis, Signal Transduction, Action. New York: Springer Dordrecht Heidelberg

Kumlay, A. M. and Eryigit, T. (2011). Growth and Development Regulators in Plants: Plant Hormones. Igdır University Journal of the Institute of Science and Technology. Vol. 1, No. 2, pp. 47-56

Martinez, J. L., Petranovic, D., and Nielsen, J. (2016). Heme Metabolism in Stress Regulation and Protein Production: From Cinderella to a Key Player. Bioengineered. Vol. 7, No. 2, pp. 112-115. DOI: 10.1080/21655979.2015.1126016

Appleford, N. E. J. and Lenton, J. R. (1997). Hormonal Regulation of α-amylase Gene Expression in Germinating Wheat (Triticum aestivum) Grains. Physiologia Plantarum. Vol. 100, Issue 3, pp. 534-542. DOI: 10.1111/j.1399-3054.1997.tb03058.x

Yamaguchi, S. (2008). Gibberellin Metabolism and Its Regulation. Annual Review of Plant Biology. Vol. 59, pp. 225-251. DOI: 10.1146/annurev.arplant.59.032607.092804