BUTANOL PRODUCTION FROM SUGARCANE JUICE BY CLOSTRIDIUM SPP. IN BATCH FERMENTATION

Abstract

Butanol production from sugarcane juice by Clostridium acetobutylicum ATCC 824 was studied inbatch fermentation at 35 °C and pH 5.0. In a comparative study of fermentation performance between usingglucose medium and sugarcane juice medium at the initial reducing sugar concentration of 45 g/L, the totalsolvent concentrations of 16.62 and 7.89 g/L were obtained from the system using glucose medium andsugarcane juice medium, respectively. The inhibition of butanol fermentation at initial glucose concentrationsabove 60 g/L was reported. On the other hand, since sugarcane juice is composed predominantly of sucrose,enhancement of butanol formation and total solvent production were achieved by the utilization of sugarcanejuice medium with the initial sugar concentration of 80-90 g/L. By utilization of sugarcane juice medium at theoptimal sugar concentration (80 g/L), the total solvents of 24.73 g/L (composed of 15.35 g/L butanol, 4.58 g/Lacetone and 4.80 g/L ethanol) with the conversion yield (Yp/s) of 32.67% and 0.26 g/(L·h) production rate wasobtained.