Effective Microorganisms (EM) as Bioremediation Agent of Profenofos Pesticide Residue in Vegetable Farm Soil from Benguet Province, Philippines

Main Article Content

Maylowen P. Lumayna
Paulina A. Bawingan

Abstract

Effective microorganisms (EM) have shown remarkable adaptability and have been used in agriculture and environmental management. This study intended to provide insights into the effectiveness of EM technology in rehabilitating soils contaminated with pesticides. Specifically, the study aimed to determine the efficiency of EM in breaking down the profenofos component of Selecron® 500 EC pesticide in a controlled condition. Results show that the initial profenofos (17.2 mg kg-1) degraded in the control and experimental groups by 94.19% and 96.45%, respectively, over a 21-day laboratory experiment. EM-treated soil samples showed a significant difference from untreated samples, as revealed in the Kruskal-Wallis (p=3.55e-12) and Freidman’s tests (p=0.20). These findings enhance our understanding of EM's capabilities in pesticide remediation as well as the natural dissipation of pesticides.

Article Details

How to Cite
Lumayna, M., & Bawingan, P. . (2024). Effective Microorganisms (EM) as Bioremediation Agent of Profenofos Pesticide Residue in Vegetable Farm Soil from Benguet Province, Philippines. Applied Environmental Research, 46(4). https://doi.org/10.35762/AER.2024049
Section
Original Article

References

Lu, J. L. Analysis of Trends of the Types of Pesticide Used, Residues and Related Factors among Farmers in the Largest Vegetable Producing Area in the Philippines. Journal of Rural Medicine, 2010, 5, 2, 184–189.

Kaur, H., & Garg, H. Pesticides: Environmental Impacts and Management Strategies. Pesticides - Toxic Aspects, IntechOpen, 2014. [Online] Available from: https://www.intechopen.com/chapters/46083

Chibuike, G. U., & Obiora, S. C. Heavy Metal Polluted Soils: Effect on Plants and Bioremediation Methods. Applied and Environmental Soil Science, 2014, 1–12.

Kapeleka, J. A., Sauli, E., Sadik, O., & Ndakidemi, P. A. Co-exposure risks of pesticides residues and bacterial contamination in fresh fruits and vegetables under smallholder horticultural production systems in Tanzania. PLOS ONE, 2020, 15, 7.

Solidum, J. N. Heavy Metal Lead in Filipino Staple Food as Studied in Metro Manila, Philippines. APCBEE Procedia, 2014, 9, 102–107.

Del Prado-Lu, J. L. Insecticide Residues in Soil, Water, and Eggplant Fruits and Farmers’ Health Effects Due to Exposure to Pesticides. Environmental Health and Preventive Medicine, 2014, 20(1), 53–62.

Reyes, G. A. & Laurean, C. P. A comprehensive assessment of pesticide residues in two vegetable-farming municipalities in Benguet. Northern Luzon Research Journal, 2007, 1, 39-54.

Sayed, K., Baloo, L., & Sharma, N. K. Bioremediation of Total Petroleum Hydrocarbons (TPH) by Bioaugmentation and Biostimulation in Water with Floating Oil Spill Containment Booms as Bioreactor Basin. International Journal of Environmental Research and Public Health, 2021, 18, 5, 2226.

Wuana, R. A. & Okieimen, F. E. Heavy metals in contaminated soils: A review of sources, Chemistry, risks and best available strategies for remediation. International Scholarly Research Network Ecology, 2011.

Shamim, S. Biosorption of Heavy Metals. IntechOpen, 2018. [Online] Available from: https://www.intechopen.com/chapters/58112.

Deshmukh, R., Khardenavis, A. A., & Purohit, H. J. Diverse Metabolic Capacities of Fungi for Bioremediation. Indian Journal of Microbiology, 2016, 56, 3, 247–264.

Javaid, A. Beneficial Microorganisms for Sustainable Agriculture. Sustainable Agriculture Reviews, 2010, 347–369.

Higa, T. & Wididana, G. N. The Concept and Theories of Effective Microorganisms. University of Ryukyus, Okinawa, 2004. [Online] Available from: T (infrc.or.jp)

Iriti, M., Scarafoni, A., Pierce, S., Castorina, G., & Vitalini, S. Soil Application of Effective Microorganisms (EM) Maintains Leaf Photosynthetic Efficiency, Increases Seed Yield and Quality Traits of Bean (Phaseolus vulgaris L.) Plants Grown on Different Substrates. International Journal of Molecular Sciences, 2019, 20, 2327.

Ngilangil, L. E. & Vilar, D. A. Effective Microorganisms as Remediation for Marginal Soil in the Philippines. Chemical Engineering Transactions, 2020, 78, 253-258.

Kannan, D. & Kumar, S. V. (2012). Effective microorganisms are used in the domestic effluent treatment system. Balkan Water Observation and Information System, 2012.

Lavanya, V. & Kannan, D. Effective Microorganisms used in Domestic Effluent Treatment System. Journal of Applied Sciences, 2019, 19, 3, 188-198.

Sree, K. D. S., Dash, S.N. & Leelavathi, A. Biological Remediation of the Municipal Solid Waste Leachate - A Case Study of Hyderabad Integrated MSW Limited. Nature Environment and Pollution Technology, 2020, 19, 1, 379-383.

Abdel-Megeed, A. & El-Nakieb, F. A. Bioremediation of pesticides in soil will significantly help reduce pollutants and remove residues that vegetables can absorb. Terrestrial and Aquatic Environmental Toxicology, 2008, 2, 1, 1-4

Ramos, M. A. G., & Yoshioka, S. A. Bioremediation of herbicide velpar K® in vitro in aqueous solution with application of EM-4 (effective microorganisms). Brazilian Archives of Biology and Technology, 2012, 55, 1, 145–149.

Fernandez-Lopez, M. G., Popoca-Ursino, C., Sanchez-Salinas, E., Tinoco-Valencia, R., Folch-Mallol, J. L., Dantan-Gonzalez, E., & Laura Ortiz-Hernandez, M. Enhancing methyl parathion degradation by the immobilization of Burkholderia sp. isolated from agricultural soils. Microbiology Open, 2017, 6, 5.

Ali, D., Nagpure, N. S., Kumar, S., Kumar, R., Kushwaha, B., & Lakra, W. S. Assessment of genotoxic and mutagenic effects of chlorpyrifos in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Food and Chemical Toxicology, 2009, 47, 3, 650–656.

He, J., Fan, M., & Liu, X. Environmental Behavior of Profenofos Under Paddy Field Conditions. Bulletin of Environmental Contamination and Toxicology, 2010, 84, 6, 771–774.

Gupta, S., Gajbhiye, V. T., Sharma, R. K., & Gupta, R. K. Dissipation of cypermethrin, chlorpyriphos, and profenofos in tomato fruits and soil following application of pre-mix formulations. Environmental Monitoring and Assessment, 2010, 174, 1-4.

Ployckuhanl, C., Vangnai, A. S., Wantala, K., & Siripattanakul‐Ratpukdi, S. Characterization of profenofos degradation by Pseudomonas plecoglossicida strain PF1 using surface response methodology. Desalination and Water Treatment, 2017, 89, 142–149.

Henry, M. C. The dissipation of profenofos, λ-cyhalothrin, and chlorothalonil in vegetables and soil under humid tropical conditions. Masters thesis, Universiti Malaysia Sarawak, (UNIMAS), 2015. [Online] Available from: https://ir.unimas.my/id/eprint/10945/

Singh, B. K., Walker, A., Morgan, J. A. W., & Wright, D. J. Biodegradation of Chlorpyrifos by Enterobacter Strain B-14 and Its Use in Bioremediation of Contaminated Soils. Applied and Environmental Microbiology, 2004, 70(8), 4855–4863.

Liao, M., Zhang, H.J. and Xie, X.M. Isolation and identification of degradation bacteria Enterobacter aerogenes for pyrethroids pesticide residues and its degradation characteristics. PubMed, 2009, 30, 8, 2445-51.

Kumar, P., Rai, A.K., Gupta, A. et al. Pesticide-Degrading and Phosphate-Solubilizing Bacilli Isolated from Agricultural Soil of Punjab (India) Enhance Plant Growth. Microbiology, 2021, 90, 848–856.

Farhan, M., Ahmad, M., Kanwal, A. Butt, Z. A., Khan, Q. F., Raza, S. A., Qayyum, H. and Wahid, A. Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies. Science Reports, 2021, 11, 10320.

Mahajan, R., Verma, S. and Chatterjee, S. Biodegradation of organophosphorus pesticide profenofos by the bacterium Bacillus sp. PF1 and elucidation of initial degradation pathway. Environmental Technology, 2023, 44, 4.

Sirajuddin, S., Khan, M. A., Qader, S. A. U., Iqbal, S., Sattar, H., & Ansari, A. A comparative study on degradation of complex malathion organophosphate using Escherichia coli IES-02 and a novel carboxylesterase. International Journal of Biological Macromolecules, 2020, 145, 445-455.

Singh, B. Organophosphorus-degrading bacteria: ecology and industrial applications. Nature Reviews Microbiology, 2007 7, 156–164.

Amstrup, S.K., Ong, S.C., Sofos, N. et al. Structural remodelling of the carbon–phosphorus lyase machinery by a dual ABC ATPase. Nature Communications, 2023, 14, 1001.

Yuan, S., Li, C., Yu, H., Xie, Y., Guo, Y., & Yao, W. Selective uptake determines the variation in degradation of organophosphorus pesticides by Lactobacillus plantarum: food Chemistry, 2021, 360, 130106.

Kumral, A. Y., Kumral, N. A., Kolcu, A., Maden, B., & Artik, B. Simulation Study for the Degradation of Some Insecticides during Different Black Table Olive Processes. American Chemical Society Omega, 2020, 5, 23, 14164–14172.

Wu, P., Chen, Z., Zhang, Y., Wang, Y., Zhu, F., Cao, B., et al. Rhodopseudomonas palustris Wastewater Treatment: Cyhalofop-Butyl Removal, Biochemicals Production and Mathematical Model Establishment. Bioresource Technology, 2019. 282, 390–397.

Xu, Y., Liu, C., Bao, J., Zhu, H, Chen, Y., Luo, Y. and Zhang, L. Microbial diversity and physicochemical properties in farmland soils amended by effective microorganisms and fulvic acid for cropping Asian ginseng. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2022, 50, 1, 12563.

Higa, T & Wididana, G. N. (2004b) Changes in the soil microflora induced by effective microorganism. International Nature Farming Research Center, 2004. [Online] Available from: https://www.infrc.or.jp/knf/1st_Conf_S_5_6.html