Extraction and Antioxidant Activity of Bioactive Compounds from Marine Microalgae Tetraselmis gracilis BIMS-PP017
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Abstract
In this study, naturally occurring bioactive compounds from marine microalgae Tetraselmis gracilis BIMS-PP017 were investigated. The Bligh and Dyer method was used for lipid extraction. Crude extracts of T. gracilis were prepared using hot water, 95% ethyl alcohol and Dichloromethane/Methanol (2:1 v/v) as extraction solvent. Analysis of total phenolic compounds was carried out using Folin-Ciocalteu method and flavonoids were quantified by aluminum chloride colorimetric method. Results indicated that T. gracilis’s Dichloromethane/Methanol hydrolysate (pellet) exhibited the highest polyphenolic content (29.69 mg GAE/gDW) whereas hot water extract (pellet) had the most flavonoid content (95.67 mg QE/gDW). Antioxidant capacity of all extracts was analyzed by DPPH assay, ABTS assay and Potassium ferricyanide reducing power assay. The results revealed that crude ethanol extract (supernatant) showed highest antioxidant activity with DPPH assay (6.03 ± 0.22%nhibition) as well as ABTS assay (88.95 ± 1.44% inhibition). The crude ethanol extract (supernatant) showed the highest reducing ability (Vitamin C equivalent = 343 ± 13.99 µg/ml). The anti-tyrosinase activity was also studied using Dopachrome method. The results suggested that Dichloromethane/Methanol extract possesses the highest inhibitory effect (38.77 ± 0.90% inhibition). Results from this study suggested that T. gracilis could potentially be a valuable source for bio-ingredients for cosmeceutical and nutraceutical applications.
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