Anti-melanogenic Activity of Tecoma stans (L.) Juss. ex Kunth Flower Extract in B16F10 Melanoma Cell Line
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Abstract
Yellow bells (Tecoma stans (L.) Juss. ex Kunth) is a widespread ornamental plant extensively cultivated in tropical regions, including Thailand. It has potential therapeutic properties due to the presence of various bioactive compounds with many biological effects, such as anticancer, antidiabetic, antibacterial, and antioxidative activities. However, anti-melanogenic and anti-tyrosinase activities of yellow bell plant extract have not been previously explored. In this study, cytotoxicity of ethyl acetate extract of yellow bell flower towards murine B16F10 skin melanoma cell line was assessed using MTT assay. The flower extracts were also tested for their inhibitory effect on melanogenesis in B16F10 cells compared with kojic acid. Tyrosinase inhibition activity was evaluated using the dopachrome method with L-tyrosine as a substrate. After treatment with 0.05 – 3.2 mg/ml yellow bell flower extracts for 48 h, the viability of B16F10 cells was found to be higher than the 90% viability at 0.05 – 0.8 mg/ml, indicating that these concentrations showed no significant toxicity as compared with the control group. The yellow flower extract at 0.8 mg/ml also effectively decreased both extracellular and intracellular melanin contents in B16F10 cells and could inhibit mushroom tyrosinase activity in vitro by 60% compared with the control. This work has shown for the first time that yellow bell flower extract have anti-melanogenic properties without causing cytotoxicity. Thus, yellow bell flower extract can potentially be used as a source for the development of skin whitening agents in the future.
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