Preliminary Report: Using Vero Cell Line for Newcastle Disease Virus (NDV) Isolation

Abstract

The preliminary study was isolation of Newcastle disease virus (NDV) using Vero Cell line from infected chickens. The aim of this study is for viral identification. The specimens of NDV were brought to Virus Laboratory, Faculty of Veterinary Medicine, Kasetsart University, Kamphaengsaen, Nakornprathom, Thailand. The comparison of virus isolation was performed using Vero Cell line, primary cell culture as Chicken Embryo Fibroblast (CEF) and chicken embryonated egg which can cause embryonic death. Virus concentration was determined. Both of the inoculated CEF and inoculated embryonated egg yielded 128 HA unit while Vero Cell line as host for NDV isolation and CEF showed the cell differentiation and Cytophathic effect (CPE) of the obvious rounded up cells which indicated the virus isolation and identification for causative agent detection. The virus concentration from infected Vero Cell line was 10 ^3.5TCID50/ml and 8 HA unit. The virus yield was then used to inoculate the primary cell culture CEF and the viral concentration was increasing to 10 ^8.75TCID50 /ml. This is the evident of being host of choice of the primary cell culture as embryonated egg for NDV isolation. However Vero Cell line could be the alternate host of choice in substitution to using the primary cell culture which has the limit of life span and more complicated preparation for the advantage of viral identification purpose of routine application.