Evaluation of Drought and Salinity Tolerance at Seedling Stage of Introgression Lines of ‘KDML105’ Rice Carrying Drought Tolerance QTL and SKC1 Salt Tolerance Gene
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Abstract
The salinity and drought tolerance levels were evaluated in twenty-two backcross improved rice lines carrying drought tolerance quantitative trait loci on chromosome 8 (DT-QTL8) and the salt tolerance SKC1 gene in the genetic background of the Thai elite rice cultivar ‘KDML105’. Firstly, drought stress was imposed on seedlings of the 22 rice lines grown in hydroponic culture by adding 20% polyethylene glycol 6000 (PEG6000), and drought tolerance was evaluated by leaf rolling, leaf drying, and biomass reduction. Ten rice lines that exhibited lower decrement in dry biomass than ‘KDML105’ after 28 days of drought stress, namely L13, L7, L24, L3, L14, L17, L8, L4, L6, and L16, were then selected for evaluation of salt tolerance in hydroponic culture in the presence of 150 mM NaCl. After 21 days of salt stress, all ten rice lines exhibited lower salt injury scores, lower Na+/K+ ratios, and lower biomass reductions than ‘KDML105’. The most tolerant line, L13, exhibited 62.58% and 47.71% reduction in dry biomass under drought and salt stress, respectively, compared with 77.35% and 83.64% reduction in ‘KDML105’. These improved lines may be tested for enhanced production in field conditions and serve as potentially good genetic resources for further improvement of ‘KDML105’ rice to tolerate multiple stresses.
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