Karyotypes of three grasshopper species (Othoptera: Acrididae/Pyrgomorphidae) in Udon Thani province

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Published: Nov 28, 2022
Keywords:
Karyotype Grasshopper Acrididae Pyrgomorphidae

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Thaninatphasit Sangpakdee
Faculty of Environmental Culture and Ecotourism, Srinakharinwirot University, Bangkok, 10110, Thailand
Pattarawit Rukthong
Department of Pharmaceutical technology, Faculty of Pharmacy, Srinakarinwirot University, Nakornnayok, 26120, Thailand
Wiwat Sangpakdee
Biology program, Faculty of Science, Udon Thani Rajabhat University, Muang, Udon Thani, 40001, Thailand

Abstract

This research aimed to examine karyotypes of some grasshopper species in the family Acrididae and Pyrgomorphidae. Ten adult male specimens of each species were collected in the area of Samprao campus, Udon Thani Rajabhat University. The metaphase chromosomes were prepared from mitotic dividing cells in testes (spermatogonia). Conventional staining was applied using 10% Giemsa’s solution. The results showed that white-tipped grasshopper (Phlaeoba antennata) had a diploid chromosome number of 2n = 23. Karyotype was consisted of all telocentric chromosomes, and karyotype formula was 2n (23) = Lt8+Mt2+St12+XO. Testaceous oblique-faced grasshopper (P. infumata) had a diploid chromosome number of 2n = 23, karyotype comprised all telocentric chromosomes, and karyotype formula was 2n (23) = Lt10+Mt8+St4+XO. Orange-winged slant-faced grasshopper (Tagasta marginella) had a diploid chromosome number of 2n = 19, karyotype was composed of all telocentric chromosomes, and karyotype formula was 2n (19) = Lt6+Mt10+St2+XO. Sex determination of these grasshopper species was XX and XO system. These cytogenetic data can be employed as a source of taxonomic information which is provide more details for evolutionary relationship knowledge of these insect groups in the future.

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Sangpakdee , T. ., Rukthong , P. ., & Sangpakdee, W. . (2022). Karyotypes of three grasshopper species (Othoptera: Acrididae/Pyrgomorphidae) in Udon Thani province. KKU Science Journal, 49(2), 194–202. Retrieved from https://ph01.tci-thaijo.org/index.php/KKUSciJ/article/view/250259
Issue
Vol. 49 No. 2 (2021): April - June 2021
Section
Research Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biography

Pattarawit Rukthong , Department of Pharmaceutical technology, Faculty of Pharmacy, Srinakarinwirot University, Nakornnayok, 26120, Thailand

Excellent Center of Integrated Molecular Pharmacokinetics for Research and Development of New Herbal and Industrial Crops Innovations (CIMPRONIC), Srinakarinwirot University, Nakornnayok 26120

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