The first karyotypic study of false ornate narrow-mouthed frog (Micryletta inornata) in Thailand

Abstract

This study is the first report on karyological analysis of the false ornate narrow-mouthed frog, Micryletta inornata. Five males and five females were collected from Chiang Mai University Hariphunchai Education Centre, Lamphun Province. Chromosomes were directly prepared from bone marrow. The cells were harvested by colchicine-hypotonic-fixation-air drying method. Conventional staining was applied to stain the chromosome using Giemsa’s solution. The results showed that the diploid chromosome number was 2n=26, the fundamental number (NF) was 56 in both male and female. The karyotype consisted of 6 large metacentric, 4 large submetacentric, 8 small metacentric and 8 small submetacentric chromosomes. No cytologically distinguishable sex chromosome was observed. The karyotype is as follows: 2n (26) = L^m₆+L^sm₄+S^m₈+S^sm₈ .