Growth and Development of Dendrobium chrysotoxum Lindl. Plantlet with Low Cost Plant Tissue Cultures Technique

Abstract

Micropropagation of Dendrobium chrysotoxum plantlet by simple plant tissue culture technique were investagated. In vitro plantlets were cultured on Vacin and Went (VW) agar medium compared with various concentrations of 0, 1/5, 2/5, 3/5, 4/5 and full strength Kittisak (2013) agar media, media from hydroponic solutions, associated with 20 g/L sugar, 7 g/L agar and pH was adjusted to 4.9 which were sterilized with 0.5 mL/L Haiter supplementation. They were incubated in 16 h photoperiod and a light intensity of 2,000 lux at 25±2 ºC compared with room temperature for 4 weeks. It was found that shoot high of plantlet cultured on KS medium with room temperature (2.33 cm) higher that VW medium with significantly. Furthermore, leaf length of this treatment (2.83 cm) also higher that all treatments with significantly. Therefore, KS medium can be used as a replacement for VW medium because it had positive effects on plant growth and development, low cost and simple preparation.  Furthermore, the result indicated that room temperature promote better growth than in the controlled temperature room. All treatments cultured at room temperature showed 100% root induction. Therefore, room temperature was the cost saving alternative for plant tissue culture.