Cosmeceutical effects of Dipterocarpus alatus bark extract via collagen synthesis stimulation of dermal fibroblast

Abstract

This research aimed to investigate cytotoxic and cosmeceutical effect of Dipterocarpus alatus Roxb. (Yang-na in Thai) bark extract, the influencing on cell viability by using MTT assay and in collagen synthesis of dermal fibroblasts by using a collagen dye test, and potential on wound healing using an in vitro cell migration model.The potential of Yang-na bark extract concentration 100 µg/mL was demonstrated, the increasing of fibroblast cell proliferation and synthesized collagen content as high as 161.04 - 282.57% and 103.69 – 136.81% of control, respectively. Accordantly, wound healing effect of Yang-na extract was manifested in reduction of wound-mimic scratch lesion (width of 59.50 – 71.35% of control) at 48 h after Yang-na treatment. This was found in similar manner as gallic acid at concentration of 5 µg/mL, the standard reference (scratch lesion width of 64.53% of control). The results exhibited the beneficial biological properties of Yang-na bark extract concentration 100 µg/mL in wound healing process with mechanisms related stimulation of cell proliferation and collagen synthesis in dermal fibroblasts. The obtained information will be useful for further research and development for substantial utilization of Yang-na bark extract in medical and aesthetical anti-aging purposes.