Total Phenolic Content and Antioxidant Activities of Aqueous Extract from Ko klan Remedy

Abstract

Ko klan remedy is folklore medicine inherit from Thai ancestor which have been used to healing since the past until now. The drugs have been locally used in an herbal remedy for the relief of muscle pain. Aims of this study were evaluated to total phenolic content and antioxidant activities of aqueous extract from Ko klan Remedy. The antioxidations were tested using by Ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging and 2,2 -azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS⁺) assay. The ko klan recipe will be consist of 3 formulas. The study showed that 1^st formula was higher content of total phenolic compounds which drugs composed with Mallotus repandus: Rhinacanthus nasutus: Elephantopus scaber: Aegle marmelos: 1: 1:1:1 (w:w:w:w). 2^nd formula which composes with Mallotus repandus: Rhinacanthus nasutus: Elephantopus scaber: Aegle marmelos; 5:2.5:1.5:1.5 (w:w:w:w) was highest antioxidant activity by ABTS assay (IC₅₀ = 0.002±0.00001 mg/mL). 3^rd formula were highest reducing by FRAP assay (29.1053±0.2362 mgTE/gEtx) and free radical scavenging by DPPH assay (IC₅₀ = 0.011±0.00004 mg/mL) which drugs contained with Mallotus repandus: Rhinacanthus nasutus: Elephantopus scabe: Ceasalpinia sappan: Cryptolepis buchanani: Piper interruptum; 2:1:1:2:2:2 (w:w:w:w). Moreover, the results found that the three formulas were antioxidation higher than Ascorbic acid and Trolox as standard substances. Further study, chemical composition(s) and pharmaceutical activity were clarified to develop medicinal plant usage.