Cytogenetics of Giant Gourami (Osphronemus goramy) using Giemsa’s Staining

Abstract

Cytogenetics of giant gourami (Osphronemus goramy) collected from natural habitat in Ubon Ratchathani province was studied. Twenty individuals comprising of 10 male and 10 female fish were used as analyzed specimens. Chromosomes were prepared from kidney and gill tissues by direct method. Twenty percent Giemsa’s staining was applied to stain the mitotic chromosomes. The standardized karyotype and idiogram were the evaluated. The results showed that diploid chromosome of O. goramy was 2n=48. The autosomes consist of 32 large telocentric, 14 medium telocentric, 2 small telocentric chromosomes. Fundamental number (NF) was 48 both of male and female. There was no significant difference in sex chromosome. The karyotype formula is as follows: 
2n = 48 = L^t₃₂ + M^t₁₄ + S^t₂