Screening of plant growth promoting bacteria from improving soil for growing plants

Abstract

Plant growth promoting bacteria (PGPB) are the soil microbes that directly stimulated growth of a plant by assisting on recycling of nutrients (nitrogen fixation, phosphate and potassium solubilization) and indirect mechanisms by being as bacterial antagonist to fungal pathogens for controlling plant diseases. The aim of this study was to screen PGPB from seven improving soil brands. 23 isolates which had plant growth promoting properties were found in the direct screening mechanism of PGPB by testing on Ashby’s agar, Pikovskaya's agar and Aleksandrov’s agar. Then indirect screening mechanism of PGPB by dual culture method was made two isolates (B-2 and B-9), they had shown the antagonistic activity to inhibit Sclerotium sp. that cause root rot disease. These two antagonist isolates were screened from improving soil B brand. Moreover, microbiological, chemical and physical property studies of improving soil B brand was found. pH was 7.77. Temperature was 26.03 °C. Moisture content was 14-31%. Electrical conductivity (EC) was 2.47 dS/m. Bacterial population was 7.49 logCFU/g. The texture was black loamy soils. These data were from the preliminary work and two isolates had the great plant growth promoting properties and were able to use for biofertilizer production.