Effects of TDZ and 2,4-D on Growth and Development of In Vitro Seedling Culture of Dendrobium formosum Roxb. ex Lindl.

Abstract

In vitro seedling culture of D. formosum on semi-solid Murashige and Skoog (MS) (1962) medium supplemented with combination of TDZ at 0.1 0.5 1.0 or 2.0 mg/L and 2,4-D at 0.1 0.5 1.0 or 2.0 mg/L compared with control for 12 weeks. The results showed that the highest percentage of shoot formation (57%), shoot numbers (1.68 shoots/plant), leaf numbers (5.70 leaves/plant) and shoots length (1.16 cm/plant) were obtained when cultured on the medium with 0.5 mg/L TDZ and 0.1 mg/L 2,4-D. However, seedlings cultured on semi-solid MS medium (control) could stimulated better shoot (2.32 shoots/plant) and leaves numbers (10.20 leaves/plant). The complete plantlets were successfully transplanted to the greenhouse and showed a satisfactory survival rate (58%) after 8 weeks when a chopped coconut husks were used as planting materials.