Cytotoxic activity against B16F10 metastatic melanoma cells of Artocarpus lakoocha root

Abstract

Artocarpus lakoocha is a popular Thai medicinal plant for health benefit, in particular the herbal cosmetic ability for nourishing and whitening skin. Preliminary in vitro cytotoxic screening against the B16F10 metastatic melanoma cells showed that the root bark extract of this plant species was 8-fold more potent than that of the heart root. Further phytochemical investigation of the more active EtOAc soluble fraction led to isolate and identify the two major types of compounds, artolakoochol (1) and oxyresveratrol (2), based on their spectroscopic data analyses. The isolates 1 and 2 exhibited inhibitory property on B16F10 cells with respective IC₅₀ values of 5.96±0.068 and 41.48±5.14 mg/mL. This is the first report on inhibitory effect against B16F10 metastatic melanoma cells of the root extracts and their major constituents provides additional valuable information towards the herbal property of A. lakoocha.