Utilization of Hydroponics Nutrient Solution to Replace Nutrient in Hippeastrum johnsonii Tissue Culture Media

Abstract

Utilization of hydroponics nutrient solution to replace nutrient in Hippeastrum johnsonii tissue culture media were studied. The bulb scale explants of H. johnsonii were cultured on agar media containing hydroponic solutions of two stocks A and B each of the same concentrations of 1, 2, 3, 4 and 5 ml/L with 30 g/L of sucrose. The pH of the medium was adjusted to 5.7 and 7 g/L of agar was added before heating until all agar was dissolved. It was then allowed to cool to 60°C and 0.1 ml/L of haiter was added. The explants were incubated at 25±2°C with light condition 16 h per day for 6 weeks. The results showed that there were no significantly differences in shoot number shoot length and root number among bulb scale explants cultured at the concentration of 3, 4 and 5 ml/L of stock A and B hydroponics media and MS medium (p<0.05) indicating that hydroponics nutrient solution can be used as a replacement for essential nutrients in H. johnsonii tissue culture media.