Lupane- and Ceanothane-Type Triterpenes from Stem Barks of Ziziphus cambodiana Pierre with Anti-Helicobacter pylori Activity

Abstract

From the stem barks of Ziziphus cambodiana Pierre, four known triterpenes and one known phenolic derivative were isolated and identified as lupeol (1), belulin (2), betulinic acid (3), ceanothic acid (4) and p-hydroxybenzoic acid (5). Their structures were mainly elucidated by NMR data analysis and by comparison of their spectroscopic data with the reported values. In addition, evaluation for antibacterial activity against H. pylori of the major isolated compounds was also investigated. The results from Broth micro dilution method showed that ceanothic acid (4) exhibited highest inhibitory activity against H. pylori DMST20165 with MIC value of 16.03 M,
which was approximately 2.6 times greater than that of the standard drug amoxicillin, but displayed weak inhibitory activity against H. pylori HP40 with MIC value of 64.22 M. This is the first report of the antibacterial activity against H. pylori of lupane- and ceanothane-type triterpenes.