Anti-Streptococcus mutans activity of lupinifolin from ethanol extract of Albizia myriophylla Benth.

Abstract

Albizia myriophylla Benth. is a medicinal plant composition in herbal formula used for remedy of toothache caused by dental caries. Previous research has revealed that such formula has antibacterial activity against Streptococcus mutans, the principal causative pathogen of tooth decay. Some substances belonging to flavonoids are shown to be the active ingredients in this plant species of which lupinifolin in particular has very good anti-S. mutans activity. In this study, anti-S. mutans activity of lupinifolin from ethanol extracts of three different collections of A. myriophylla cultivated in Thailand including those from herb shop in Songkhla (JB01), Hat Yai district of Songkhla (JB02), and Wang Nam Yen district of Sakaeo was carried out using broth microdilution. The quantitative test of lupinifolin from ethanol extracts of these 3 collections of A. myriophylla was performed herein by High Performance Liquid Chromatography.The result showed that each of the samples tested had the difference of lupinifolin content. The ethanolic wood extracts from 3 collections of A. myriophylla including JB01, JB02, and JB03 have 93.85, 57.81, and 0.04 mg/g of lupinifolin, respectively. The minimum inhibitory concentrations (MICs) of JB01 JB02 and JB03 against S. mutans were 3.9, 31.25, and 500 µg/ml, respectively. JB01 containing the maximum quantity of lupinifolin showed the best activity while JB03 containing the minimum quantity of
lupinifolin exhibited the lowest activity. Lupinifolin may be used as a marker compound of A. myriophylla for quality control of its raw materials as well as its herbal medicinal products in the future.