Karyotype of Large Flying Fox (Pteropus vampyrus) in Thailand

Abstract

This work was the description of a karyotype study of the large flying fox, Pteropus vampyrus (Chiroptera, Pteropodidae). Blood samples were taken from two males and two females were kept in Loung temple, Watloung sub-district, Panutnikom district, Chonburi province. The chromosome was prepared by lymphocyte cell culture technique. The cultured cells were harvested by colchicine-hypotonic-fixation-air drying. Conventional staining method was applied to stain the chromosome using Giemsa’s solution. The results showed that the number of diploid chromosomes of large flying fox was 2n=38, the fundamental number (NF)
was 72 both male and female. The types of autosomes were 8 large metacentric, 8 large submetacentric, 2 medium submetacentric, 14 small submetacentric and 4 small acrocentric chromosomes. The X chromosome was the large metacentric chromosome and the Y chromosome was the smallest telocentric chromosome. These results will be useful for future studies of conservation, chromosome evolution and basic genetics information in these bats. The karyotype formula for the large flying fox is as follows:
2n (38) = L^m₈+L^sm₈+M^sm₂+S^sm₁₄+S^a₄ + โครโมโซมเพศ (XX/XY)