Development of Agrobacterium-Mediated Transformation in Orchid

Abstract

The objective of this research was to establish gene transformation procedure for genus Dendrobium by using Agrobacterium tumefaciens AGLO (pBI121 or pSCV1.6). The dihydroflavonol 4- reductase gene (DFR) were constructed in plasmid pBI121 or pSCV1.6 which containing gus gene and kanamycin resistance gene as reporter gene. The positive results of GUS assay revealed the GUS activity blue while the expression level of transcription in the technical was inspected by RT-PCR