Growth and effective inhibition of pathogens of Cordyceps militaris cultured on Sakon Nakhon local rice
DOI:
https://doi.org/10.55674/cs.v17i2.261051Keywords:
Cordyceps militaris, Pathogenic microorganisms, Local rice, Antimicrobial activityAbstract
This study investigated the growth and antimicrobial activity of Cordyceps militaris cultivated on two media types—Formula 1 (glucose-based) and Formula 2 (sucrose-based)—using five local rice varieties from Sakon Nakhon Province: Hom Nang Nual, Red Jasmine, Red Sticky, Kham Noi, and Kham Bai Kiew rice. The growth experiment, spanning 70 days, was divided into three stages, during which the fresh and dry weights of fruiting bodies and bases were recorded. Results showed that Formula 2 with Red Jasmine rice supported the highest growth, yielding 9.65 ± 1.23 g (fresh) and 1.54 ± 0.05 g (dry) per bottle. In Formula 1, the highest yield was observed using Kham Noi rice, with 9.62 ± 1.01 g (fresh) and 0.71 ± 0.16 g (dry) per bottle. For antimicrobial testing, dried C. militaris samples were soaked in 95% ethanol for 48 h, filtered, and concentrated via rotary evaporation at 50 °C. The crude extracts were tested against Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus, and Escherichia coli using the disc diffusion method at concentrations of 20,000 – 100,000 ppm. At 100,000 ppm, all extracts exhibited antibacterial activity. Notably, the extract from Red Jasmine rice in Formula 1 showed the highest inhibition against E. coli, with a clear zone of 12.50 ± 5.91 mm. Statistical analysis confirmed significant differences (p ≤ 0.01) in inhibitory effectiveness. These findings indicate that a sucrose-based medium enhances the yield and quality of C. militaris, and nutrient-rich local rice substrates contribute to its antimicrobial potential. The study supports the application of local rice in C. militaris cultivation and suggests potential for developing natural antimicrobial products for future medical use.
GRAPHICAL ABSTRACT
HIGHLIGHTS
- Sucrose-based medium with Red Jasmine rice yielded the highest growth of Cordyceps militaris in both fresh and dry weights.
- The crude extract from C. militaris cultivated on Red Jasmine rice demonstrated the strongest inhibition against E. coli, showing the largest inhibition zone.
- Local rice varieties from Sakon Nakhon demonstrated strong potential as substrates for cultivating C. militaris for natural antimicrobial product development.
References
S.K. Das, M. Masuda, A. Sakurai, M. Sakakibara, Medicinal uses of the mushroom Cordyceps militaris, Fitoterapia. 81(8) (2010) 961-968. 10.1016/j.fitote.2010.07.010.
P. Ratnasajja, S. Taikhaw, Effects of crude extract in stroma of Cordyceps militaris for human enteropathogenic bacterial inhibition, J. Res. Unit Sci. Technol. Environ., 15(1) (2024) 96-113.
C.H. Dong, T. Yang, T. Lian, A comparative study of the antimicrobial, antioxidant, and cytotoxic activities of methanol extracts from fruit bodies and fermented mycelia of caterpillar medicinal mushroom Cordyceps militaris (Ascomycetes), Int J Med Mushrooms. 16(5) (2014) 485-495.10.1615/intjmedmushrooms.v16.i5.70.
S.L. Qu, S.S. Li, D. Li, P.J. Zhao, Metabolites and Their Bioactivities from the Genus Cordyceps, MDPI. 10(8) (2022) 1-20. 10.3390/microorganisms10081489.
D. Zhang, Q. Tang, X. He, Y. Wang, G. Zhu, L. Yu, Antimicrobial, antioxidant, anti-inflammatory, and cytotoxic activities of Cordyceps militaris spent substrate, PLoS ONE. 18(9) (2023) 1-23. 10.1371/journal.pone.0291363.
L. Ma, S. Zhang, M. Du, Cordycepin from Cordyceps militaris prevents hyperglycemia in alloxan-induced diabetic mice, Nutr Res. 35(5) (2015) 431-9. 10.1016/j.nutres.2015.04.011.
Y. Tongaram, A comparative study of Cordyceps militaris (Tung Chao) culture from liquid and solid inoculation on growth medium mixed with Thai millet, CRMA Journal. 13(1) (2015) 87-99.
M.Y. Chang, G.J. Tsai, J.Y. Houng, Optimization of the medium composition for the submerged culture of Ganoderma lucidum by Taguchi array design and steepest ascent method, Enzyme and Microb. Technol. 38(3-4) (2006) 407-414. 10.1016/j.enzmictec.2005.06.011.
Thai Rice Exporters Association, Rice export report, http://www.thairiceexporters.or.th/, 22 January 2025.
T. Thapingkae, M. Yachai, S. Sritiwong, K. Upalsilp, A. Phonpanawit, A. Thongthap, W. Thapingkae, Study on the cultivation of Golden Cordyceps mushroom and its applications, Research report, Chiang Mai Rajabhat University, Chiang Mai, 2014.
BCG model, https://www.doae.go.th/en/, 21 January 2025.
B. Shrestha, W. Zhang, Y. Zhang, X. Liu, The medicinal fungus Cordyceps militaris: Research and development, Mycol. Prog. 11 (2012) 599-614.10.1007/s11557-012-0825-y.
S. Tasun, Optimization condition for Cordyceps militaris cultivation on spent coffee grounds, Master's thesis, Mahasarakham University, Mahasarakham, 2023.
A. Imtiaj, T. S. Lee, Screening of antifungal activities from Korean wild mushrooms, World J. Agric. Sci. 3(3) (2007) 316-321.
S. Awarun, Suitable Media for Cultivation of Cordyceps militaris, JVIA. 3(2) (2019) 15-23.
H.N. Dang, C.L, Wang, H.L. Lay, Effect of nutrition, vitamin, grains, and temperature on the mycelium growth and antioxidant capacity of Cordyceps militaris (strains AG-1 and PSJ-1), J RADIAT RES APPL SC. 11(2) (2017) 130-138. 10.1016/j.jrras.2017.11.003
P. Ruttanasutja, S. Taikhao, Effects of crude extract in stroma of Cordyceps militaris for human enteropathogenic bacterial inhibition, J. Res. Unit Sci. Technol. Environ. 15(1) (2024). 96-113.10.14456/jstel.2024.8.
Z.C. Liang, C.H. Liang, C.Y. Wu, Various Grain Substrates for the Production of Fruiting Bodies and Bioactive Compounds of the Medicinal Caterpillar Mushroom, Cordyceps militaris (Ascomycetes), Int. J. Med. Mushrooms. 16 (6) (2014) 569-578.10.1615/IntJMedMushrooms.v16.i6.60.
R. Sornprasert, P. Saenkamon, C. Chanbamrung, M. Sanguankaew, A. Hambananda, Cultivation of Cordyceps militaris (L.) Link BCC 18247 mycelium with different grains, Chandrakasem Rajabhat University Journal. 18 (35) (2012) 83-91.
R. Sornprasert, A. Hambananda, S. Aroonsrimorakot, Cultivation of Cordyceps militaria using different cereal grains and local insects and inhibitory efficiency against Trichophyton rubrum and Staphylococcus aureus, The Journal of KMUTNB, 26(2) (2016) 239-248.
N. Srijumpa, R. Norarat, S.N. Nan, T. Maneerat, Effect of Different LED Color on Yield and Cordycepin Production of Cordyceps militaris, Thai J. Agric. Sci. 40 (1) (2022) 81-89.
X.B. Mao, T. Eksriwong, S. Chauvatcharin, J. J. Zhong, Optimization of carbon source and C:N ratio for cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris, Process Biochem. 40(5) (2005) 1667-1672. 10.1016/j.procbio.2004.06.046.
W. Palakawong, Effect of carbon and protien sources in liquid to static cultivation of Cordyceps militaris, Bachelor of Science in Biotechnology, Thammasat University, Bangkok, 2018.
N. Deshmukh, L. Bhaskaran, Comparative analysis of the antioxidant and antibacterial activity of methanolic mycelium extract from Cordyceps militaris: A comprehensive study, Biological Forum. 15(5) (2023) 1681-1686.
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