Application of Cytochrome P 450 (CYP1A) as Biomarker in Fish to Evaluate Aquatic Contamination and its Current Status in Thailand
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Keywords:
Bioindicator Benzo[a]pyrene Monooxygenase Xenobiotic
Main Article Content
Abstract
Abstract
Cytochrome P450 (CYP1A) is monooxygenases enzyme playing important roles in
metabolizing (activation and/or inactivation) xenobiotics in the organism which is
obtained by various routes such as ingestion or skin absorption, lung and other
epithelial layers contacting with the surrounding environment. In the aquatic
environment contamination with xenobiotics such as polynuclear aromatic hydrocarbons
(PAH), planar polychlorinated biphenyls (PCB) and dioxins, CYP1A expression in fish
can be evaluated by measuring 7-Ethoxyresorufin O-Deethylase (EROD) activity or
antibody techniques such as Western blotting, Enzyme Linked Immunosorbent Assay
(ELISA) or other immunohistochemistry. Although P450 enzymes mostly found in the
liver, they also play a role in other extra hepatic organs of vertebrates ranging from
fish to mammals. Different expression of P450 enzyme among various organs and
cell types should be concerned in studying the responses of those cells and organs to
toxicants. As the importance described above, CYP1A can be used as the useful
alternative bio-indicator to assess water pollution. In Thailand, there have been few
studies on CYP1A application as a biomarker. Thus, its efficiency in water monitoring
should be further studied.
Article Details
How to Cite
[1]
C. Thanomsit, “Application of Cytochrome P 450 (CYP1A) as Biomarker in Fish to Evaluate Aquatic Contamination and its Current Status in Thailand”, RMUTI Journal, vol. 9, no. 3, pp. 202–214, Dec. 2016.
Section
Academic article
References
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Aquatic Toxicology; Molecular, Biochemical and Cellular Perspectives. Boca Raton: CRC press. pp. 87-206
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[11] Pollenz, R.S. (2002). The Mechanism of AH Receptor Protein Down Regulation (Degradation) and its Impact on AH Receptor-Mediated Gene Regulation. Chemico-Biological Interactions. Vol. 141. pp. 41-61
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[13] Reynaud, S., Raveton, M. and Ravanel, P. (2008). Interactions Between Immune and Biotransformation Systems in Fish: A Review. Aquatic Toxicology. Vol. 87. pp. 139-145
[14] Zodrow, J.M., Stegemanand, J.J. and Tanguay, R.L. 2004. Histological Analysis of Acute Toxicity of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) in Zebrafish. Aquatic Toxicology. Vol. 66. pp. 25-38
[15] Carlson, E.A., Li, Y. and Zelikoff, J.T. (2002). The Japanese Medaka (Oryzias latipes) Model: Applicability for Investigating the Immunosuppressive Effect of the Aquatic Pollutant Bezo[a]pyrene (BaP). Marine Environmental Resource. Vol. 54. pp. 1-5
[16] Diekmann, M. and Nagel, R. (2005). Different Survival Rates in Zebrafish (Danio rerio) from Different Origins. Journal of Applied Ichthyology. Vol. 21. pp. 451-454
[17] Lewis, D.F.V., Eddershaw, P.J., Dickins, M., Tarbit, M.H. and Goldfarb, P.S. (1998). Structural Determinants of Cytochrome P450 Substrate Specificity, Binding Affinity and Catalytic Rate. Chemico-Biological Interactions. Vol. 115. pp. 175-199
[18] George, S.G. (1994). Enzymology and Molecular Biology of Phase II Xenobiotic- conjugating Enzymes in Fish. In: Malins, D.C., Ostrander, G.K. (Eds.), Aquatic Toxicology; Molecular, Biochemical and Cellular Perspectives. CRC Press, Boca Raton, pp. 37-85
[19] Wolkers, J., J rgensen, E.H., Nijmeijer, S.M. and Witkamp, R.F. (1996). Time-dependent Induction of Two Distinct Hepatic Cytochrome P4501A Catalytic Activities at Low Temperatures in Arctic Charr (Salvelinus alpinus) After Oral Exposure to Benzo(a)pyrene. Aquatic Toxicology. Vol. 35. pp. 127-138
[20] Boobis, A.R., Sesardica, D., Murray, B.P., Edwards, R.J., Singleton, A.M., Richa, K. J., Murray, S., De La Torre, R., Segura, J., Pelkonen, O., Pasanen, M., Kobayashi S., Zhi-guanga T. and Davies, D.S. (1990). Species Variation in the Response of the Cytochrome P-450-Dependent Monooxygenase System to Inducers and Inhibitors. Xenobiotica. Vol. 20. No. 11. pp. 1139-1161
[21] Smith, D.A. (1991). Species Difference In Metabolism and Pharmacokinetics: Are we Close to an Understanding?. Drug Metabolism Reviews. Vol. 23. pp. 355-373
[22] McNeill, S.A., Arens , C., Hogan , N.,K ollner, B. and R.vandenHeuvel, M. (2012). Immunological Impacts of Oil Sands-Affected Waters on Rainbow Trout Evaluated Using an in Situ Exposure. Ecotoxicology and Environmental Safety.
Vol. 84. pp. 254-261
[23] Ryvolova, M., Krizkova, S., Adam, V., Beklova, M., Trnkova, L., Hubalek, J. and Kizek, R. (2011). Analytical Methods for Metallothionein Detection. Current Analytical Chemistry. Vol. 7. pp. 243-261
[24] Sturve, J., Balk, L., Liewenborg, B., Adolfsson-Erici, M., F rlin, L. and Almroth, B.C. (2014). Effects of an Oil Spill in a Harbor Assessed Using Biomarkers of Exposure in eelpout. Environmental Science and Pollution Research. Vol. 21. pp. 13758-13768
[25] Ortiz-Delgado, J.B., Segnerand, H. and Sarasquete, C. (2009). Brain CYP1A in seabream, Sparus Aurata Exposed to Benzo(a)pyrene. Histology and Histopathology. Vol. 24. pp. 1263-1273
[26] Abrahamson, A. (2007). Gill EROD Activity in Fish: A Biomarker for Waterborne Ah-receptor Agonists. Sweden: Acta Universittalis Upsaliensis. p. 55
[27] J nsson, M.E., Gao, K., Olsson, J.A., Goldstone, J.V. and Brandt, I. (2010). Induction Patterns of New CYP1 Genes in Environmentally Exposed Rainbow Trout. Aquatic Toxicology. Vol. 98. No. 4. pp. 311-321
[28] Parente, T.E.M., Rebelo, M.F., Da-Silva, M.L., Woodin, BR., Goldstone, J.V. Bisch, P.M., Paumgartten, F.J.R. and Stegeman, J.J. (2011). Structural Features of Cytochrome P450 1A Associated with the Absence of EROD Activity in Liver of the of the Loricariid Catfish Pterygoplichthys sp. Gene. Vol. 489. No. 2. pp. 111-118
[29] Stegeman, J.J. (2000). Cytochrome P450 Gene Diversity and Function in Marine Animals: Past, Present, and Future. Marine Environmental Research. Vol. 50. No. 1-5. pp. 61-62
[30] Hassanin, A.A.I., Kaminishi1, Y., Osman, M.M.M., Abdel- Wahad, Z.H.H., El-Kady, M.A.H. and Itakur, T. (2009). Cloning and Sequence Analysis of Benzo-A-Pyreneinducible Cytochrome P450 1A in Nile tilapia (Oreochromis niloticus). African Journal of Biotechnology. Vol. 8. No. 11. pp. 2545-2553
[31] Klemz, C., Salvo, L.M., Bastos Neto, J.C., Dias Bainy, A.C. and Silva de Assis, H.C. (2010). Cytochrome P450 Detection in Liver of the Catfish Ancistrus multispinis (Osteichthyes, Loricariidae). Brazilian Archives of Biology and Rechnology. Vol. 53. No. 2. pp. 361-368
[32] Cheevaporn, V.F. and Beamish, F.B.H. (2007). Cytochrome P450 1A Activity in Liver and Fixed Wavelength Fluorescence Detection of Polycyclic Aromatic Hydrocarbons in the Bile of Tongue-Fish (Cynoglossus acrolepidotus, Bleeker) in Relation to Petroleum Hydrocarbons in the Eastern Gulf of Thailand. Journal of Environmental Biology. Vol. 28. No. 4. pp.701-705
[33] Kachanopas-Barnette, P. Mokkongpai, P.Wassmur, B. Celander, M.C. and Sawangwong, P. (2010). Molecular Characterization of Cytochrome P450 1A (CYP1A) in Asian Sea bass (Lates calcalifer Bloch) and Its Application as a
Biomarker in the Gulf of Thailand. Asian Journal of Water, Environment and Pollution. Vol. 7. No. 2. pp. 43-51
[34] Nanthanawat, P., Khongchareonporn, N, Prasatkaew, W. and Kanchanopas- Barnette, P. (2014). Production and Characterization of Monoclonal Antibody Specific to Cytochrome P4501A (CYP1A) in Asian Sea Bass (Lates calcarifer Bloch) Exposed to Benzo[a]Pyrene. Burapha Science Journal. Vol.19. No. 2. pp.1-13
[35] Ngamdee, V. and Boonphakdee, C. (2013). The Partial Structure of cyp1a Gene of Sea Bass (Lates calcarifer). Thai Journal of Genetics. Vol. 1. pp. 356-360
[2] Oost, V.D., Beyer, R., Vermeulen, J. and Nico, P.E. (2003). Fish Bioaccumulation and Biomarkers in Environmental Risk Assessment: A Review. Environmental Toxicology and Pharmacology. Vol. 13. pp. 57-14
[3] Commandeur, J.N.M., Stijntjes, G.J. and Vermeulen, N.P.E. (1995). Enzymes and Transport Systems Involved in the Formation and Disposition of Glutathione S-conjugates. Role in Bioactivation and Detoxication Mechanisms of Xenobiotics. Pharmacological Reviews. Vol. 47. pp. 271-330
[4] Goeptar, A.R., Scheerens, H. and Vermeulen, N.P.E. (1995). Oxygen Reductase and Substrate Reductase Activity of Cytochrome P450. Critical Reviews in Toxicology. Vol. 25. pp. 25-65
[5] Stegeman, J.J. and Hahn, M.E. (1994). Biochemistry and Molecular Biology of Monooxygenase: Current Perspective on Forms, Functions, and Regulation of Cytochrome P450 in Aquatic Species. In: Malins, D.C., Ostrander, G.K. (Eds.),
Aquatic Toxicology; Molecular, Biochemical and Cellular Perspectives. Boca Raton: CRC press. pp. 87-206
[6] Bucheli, T.D. and Fent, K. (1995). Induction of Cytochrome P450 as a Biomarker for Environmental Contamination in Aquatic Ecosystems. Critical Reviews in Environmental Science and Technology. Vol. 25. pp. 201-268
[7] Eggens, M.L., Bergman, A., Vethaak, D., Van der Weiden, M., Celander, M. and Boon, J.P. (1995). Cytochrome P450 1A Indices as Biomarkers of Contaminant Exposure: Results of a Field Study with Plaice, Pleuronectus Platessa and Flounder, Platichtys Flesus , from the Southern North Sea. Aquatic Toxicology. Vol. 32. pp. 211-225
[8] Walker, C.H., Hopkin, S.P., Sibly, R.M. and Peakall, D.B. (2006). Principle of Ecotoxicology. USA: Taylor and Francis
[9] Schlezinger, J.J. and Stegeman, J.J. (2001). Induction and Suppression of Cytochrome P450 1A by 3, 3%, 4,4%, 5-pentachlorobiphenyl and its Relationship to Oxidative Stress in the Marine Fish Scup (Stenotomus chrysops). Aquatic Toxicology. Vol. 52. pp. 101-115
[10] Rees, C.B., McCormick, S.D. and Li, W. (2005). A Non-Lethal Method to Estimate CYP1A Expression in Laboratory and Wild Atlantic Salmon (Salmo salar). Comparative Biochemistry and Physiology C. Vol. 141. pp. 217-224
[11] Pollenz, R.S. (2002). The Mechanism of AH Receptor Protein Down Regulation (Degradation) and its Impact on AH Receptor-Mediated Gene Regulation. Chemico-Biological Interactions. Vol. 141. pp. 41-61
[12] Hahn, M.E., Woodin, B.R., Stegeman, J.J. and Tillitt, D.E. (1998). Aryl Hydrocarbon Receptor Function in Early Vertebrates: Inducibility of Cytochrome P450 1A in Agnathan and Elasmobranch Fish. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology. Vol. 120. pp. 67-75
[13] Reynaud, S., Raveton, M. and Ravanel, P. (2008). Interactions Between Immune and Biotransformation Systems in Fish: A Review. Aquatic Toxicology. Vol. 87. pp. 139-145
[14] Zodrow, J.M., Stegemanand, J.J. and Tanguay, R.L. 2004. Histological Analysis of Acute Toxicity of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) in Zebrafish. Aquatic Toxicology. Vol. 66. pp. 25-38
[15] Carlson, E.A., Li, Y. and Zelikoff, J.T. (2002). The Japanese Medaka (Oryzias latipes) Model: Applicability for Investigating the Immunosuppressive Effect of the Aquatic Pollutant Bezo[a]pyrene (BaP). Marine Environmental Resource. Vol. 54. pp. 1-5
[16] Diekmann, M. and Nagel, R. (2005). Different Survival Rates in Zebrafish (Danio rerio) from Different Origins. Journal of Applied Ichthyology. Vol. 21. pp. 451-454
[17] Lewis, D.F.V., Eddershaw, P.J., Dickins, M., Tarbit, M.H. and Goldfarb, P.S. (1998). Structural Determinants of Cytochrome P450 Substrate Specificity, Binding Affinity and Catalytic Rate. Chemico-Biological Interactions. Vol. 115. pp. 175-199
[18] George, S.G. (1994). Enzymology and Molecular Biology of Phase II Xenobiotic- conjugating Enzymes in Fish. In: Malins, D.C., Ostrander, G.K. (Eds.), Aquatic Toxicology; Molecular, Biochemical and Cellular Perspectives. CRC Press, Boca Raton, pp. 37-85
[19] Wolkers, J., J rgensen, E.H., Nijmeijer, S.M. and Witkamp, R.F. (1996). Time-dependent Induction of Two Distinct Hepatic Cytochrome P4501A Catalytic Activities at Low Temperatures in Arctic Charr (Salvelinus alpinus) After Oral Exposure to Benzo(a)pyrene. Aquatic Toxicology. Vol. 35. pp. 127-138
[20] Boobis, A.R., Sesardica, D., Murray, B.P., Edwards, R.J., Singleton, A.M., Richa, K. J., Murray, S., De La Torre, R., Segura, J., Pelkonen, O., Pasanen, M., Kobayashi S., Zhi-guanga T. and Davies, D.S. (1990). Species Variation in the Response of the Cytochrome P-450-Dependent Monooxygenase System to Inducers and Inhibitors. Xenobiotica. Vol. 20. No. 11. pp. 1139-1161
[21] Smith, D.A. (1991). Species Difference In Metabolism and Pharmacokinetics: Are we Close to an Understanding?. Drug Metabolism Reviews. Vol. 23. pp. 355-373
[22] McNeill, S.A., Arens , C., Hogan , N.,K ollner, B. and R.vandenHeuvel, M. (2012). Immunological Impacts of Oil Sands-Affected Waters on Rainbow Trout Evaluated Using an in Situ Exposure. Ecotoxicology and Environmental Safety.
Vol. 84. pp. 254-261
[23] Ryvolova, M., Krizkova, S., Adam, V., Beklova, M., Trnkova, L., Hubalek, J. and Kizek, R. (2011). Analytical Methods for Metallothionein Detection. Current Analytical Chemistry. Vol. 7. pp. 243-261
[24] Sturve, J., Balk, L., Liewenborg, B., Adolfsson-Erici, M., F rlin, L. and Almroth, B.C. (2014). Effects of an Oil Spill in a Harbor Assessed Using Biomarkers of Exposure in eelpout. Environmental Science and Pollution Research. Vol. 21. pp. 13758-13768
[25] Ortiz-Delgado, J.B., Segnerand, H. and Sarasquete, C. (2009). Brain CYP1A in seabream, Sparus Aurata Exposed to Benzo(a)pyrene. Histology and Histopathology. Vol. 24. pp. 1263-1273
[26] Abrahamson, A. (2007). Gill EROD Activity in Fish: A Biomarker for Waterborne Ah-receptor Agonists. Sweden: Acta Universittalis Upsaliensis. p. 55
[27] J nsson, M.E., Gao, K., Olsson, J.A., Goldstone, J.V. and Brandt, I. (2010). Induction Patterns of New CYP1 Genes in Environmentally Exposed Rainbow Trout. Aquatic Toxicology. Vol. 98. No. 4. pp. 311-321
[28] Parente, T.E.M., Rebelo, M.F., Da-Silva, M.L., Woodin, BR., Goldstone, J.V. Bisch, P.M., Paumgartten, F.J.R. and Stegeman, J.J. (2011). Structural Features of Cytochrome P450 1A Associated with the Absence of EROD Activity in Liver of the of the Loricariid Catfish Pterygoplichthys sp. Gene. Vol. 489. No. 2. pp. 111-118
[29] Stegeman, J.J. (2000). Cytochrome P450 Gene Diversity and Function in Marine Animals: Past, Present, and Future. Marine Environmental Research. Vol. 50. No. 1-5. pp. 61-62
[30] Hassanin, A.A.I., Kaminishi1, Y., Osman, M.M.M., Abdel- Wahad, Z.H.H., El-Kady, M.A.H. and Itakur, T. (2009). Cloning and Sequence Analysis of Benzo-A-Pyreneinducible Cytochrome P450 1A in Nile tilapia (Oreochromis niloticus). African Journal of Biotechnology. Vol. 8. No. 11. pp. 2545-2553
[31] Klemz, C., Salvo, L.M., Bastos Neto, J.C., Dias Bainy, A.C. and Silva de Assis, H.C. (2010). Cytochrome P450 Detection in Liver of the Catfish Ancistrus multispinis (Osteichthyes, Loricariidae). Brazilian Archives of Biology and Rechnology. Vol. 53. No. 2. pp. 361-368
[32] Cheevaporn, V.F. and Beamish, F.B.H. (2007). Cytochrome P450 1A Activity in Liver and Fixed Wavelength Fluorescence Detection of Polycyclic Aromatic Hydrocarbons in the Bile of Tongue-Fish (Cynoglossus acrolepidotus, Bleeker) in Relation to Petroleum Hydrocarbons in the Eastern Gulf of Thailand. Journal of Environmental Biology. Vol. 28. No. 4. pp.701-705
[33] Kachanopas-Barnette, P. Mokkongpai, P.Wassmur, B. Celander, M.C. and Sawangwong, P. (2010). Molecular Characterization of Cytochrome P450 1A (CYP1A) in Asian Sea bass (Lates calcalifer Bloch) and Its Application as a
Biomarker in the Gulf of Thailand. Asian Journal of Water, Environment and Pollution. Vol. 7. No. 2. pp. 43-51
[34] Nanthanawat, P., Khongchareonporn, N, Prasatkaew, W. and Kanchanopas- Barnette, P. (2014). Production and Characterization of Monoclonal Antibody Specific to Cytochrome P4501A (CYP1A) in Asian Sea Bass (Lates calcarifer Bloch) Exposed to Benzo[a]Pyrene. Burapha Science Journal. Vol.19. No. 2. pp.1-13
[35] Ngamdee, V. and Boonphakdee, C. (2013). The Partial Structure of cyp1a Gene of Sea Bass (Lates calcarifer). Thai Journal of Genetics. Vol. 1. pp. 356-360