Bacteria and Nutrients in Fermented Bio-extracts from Aromatic Coconut Husk

Main Article Content

Thunchanok Thongsamer
Nareerat Sukkhee
Soydoa Vinitnantharat
Bundit Tirachulee
Pavinee Patanachan
Vassanasak Limkuansuwan

Abstract

This study aimed to analyze the characteristics of bio-extracts from discarded fresh aromatic coconut husk using different microbial seeds, super PD.2 and PD.7 for 30 days. The bio-extracts were analyzed for microbial community and the variation of pH, electrical conductivity, tannin, and nutrient composition. The results showed that the bio-extract using PD.2 predominantly contained Lactobacillales of 80 %, while the bio-extract using PD.7 contained Bacillales of 53 % and Lactobacillales of 24 %. The bio-extract contained macro and micronutrients at levels comparable to soilless plant nutrient formulas, except for nitrogen content. Moreover, tannin was detected in both bio-extracts. Therefore, the bio-extracts from coconut husks may be used in agriculture to improve soil quality. In addition, it contains anti-microbial and beneficial microorganisms.

Article Details

How to Cite
[1]
T. Thongsamer, N. Sukkhee, S. Vinitnantharat, B. Tirachulee, P. Patanachan, and V. Limkuansuwan, “Bacteria and Nutrients in Fermented Bio-extracts from Aromatic Coconut Husk”, RMUTI Journal, vol. 18, no. 2, pp. 91–102, Aug. 2025.
Section
Research article

References

Adamczyk, B., Kitunen, V. and Smolander, A. (2008). Protein Precipitation by Tannins in Soil Organic Horizon and Vegetation in Relation to Tree Species. Biology and Fertility of Soils, 45, 55-64. https://doi.org/10.1007/s00374-008-0308-0

Aguilar-Zarate, P., Cruz-Hemandez, M.A., Montanez, J.C., Belmares-Cerda, R.E. and Aguilar, C.N. (2014). Bacterial Tannases: Production, Properties and Applications Tanasas Bacterianas: Produccion, Propiedades Y Aplicaciones. Revista Mexicana de Ingenieria Quimica, 13(1), 63-74. https://www.redalyc.org/pdf/620/62031166005.pdf

Ali Al Meselmani M. (2023). Nutrient Solution for Hydroponics. In Metin Turan, Sanem Argin, Ertan Yildirim and Adem Güneş (Eds), Recent Research and Advances in Soilless Culture. (pp. 1-21).

Aroonrungsikul, C., Rattanakritakul, C., Korpraditskul R. and Romphopak T. (2004). Bio-Fermented Juice Quality and Components. Proceedings of the 42nd Kasetsart University Annual Conference (pp. 481-488). Bangkok, Thailand (in Thai)

Christensen, L.F., García-Béjar, B., Bang-Berthelsen, C.H. and Hansen, E.B. (2022). Extracellular Microbial Proteases with Specificity for Plant Proteins in Food Fermentation. International Journal of Food Microbiology, 381, 109889. https://doi.org/10.1016/j.ijfoodmicro.2022.109889

Costa, O.Y.A., Raaijmakers, J.M. and Kuramae, E.E. (2018). Microbial Extracellular Polymeric Substances: Ecological Function and Impact on Soil Aggregation. Fronteir in Microbiolology, 9, 1636. https://doi.org/10.3389/fmicb.2018.01636

Grgas, D., Rukavina, M., Bešlo, D., Štefanac, T., Crnek, V., Šikić, T., Habuda-Stanić, M. and Landeka Dragičević, T. (2023). The Bacterial Degradation of Lignin-A Review. Water, 15(7), 1272. https://doi.org/10.3390/w15071272

Hameed, A., Shahina, M., Lai, W.A., Lin, S.Y., Young, L.S., Liu, Y.C., Hsu, Y.H. and Young, C.C. (2015). Oricola cellulosilytica gen. nov., sp. nov., A Cellulose-degrading Bacterium of the Family Phyllobacteriaceae Isolated from Surface Seashore Water, and Emended Descriptions of Mesorhizobium loti and Phyllobacterium myrsinacearum. Antonie Van Leeuwenhoek, 107(3), 759-771. https://doi.org/10.1007/s10482-014-0370-6

Hussain, T., Khan, A.A. and Mohamed, H.I. (2023). Metabolites Composition of Bacillus subtilis Hussain T-AMU Determined by LC-MS and Their Effect on Fusarium Dry Rot of Potato Seed Tuber. Phyton-International Journal of Experimental Botany, 92(3), 783-799. https://doi.org/10.32604/phyton.2022.026045

Jantassana, C. and Maniin, P. (2024). Effect of Tannin Concentrate on Seed Germination of Cannabis RSU 01. Journal of Science and Technology, 13(2), 1-9. (in Thai) http://ph03.tci-thaijo.org/index.php/JSTKU/article/view/3202/2470 (in Thai)

Ji, S.H., Gururani, M.A. and Chun, S.-C. (2014). Isolation and Characterization of Plant Growth Promoting Endophytic Diazotrophic Bacteria from Korean Rice Cultivars. Microbiological Research, 169(1), 83-98. https://doi.org/10.1016/j.micres.2013.06.003

Kim, B., Park, A.R., Song, C.W., Song, H. and Kim, J.-C. (2022). Biological Control Efficacy and Action Mechanism of Klebsiella pneumoniae JCK-2201 Producing Meso-2,3- Butanediol Against Tomato Bacterial Wilt. Frontier in Microbiology, 13, 914589. https://doi.org/10.3389/fmicb.2022.914589

Kusuwanwichid, S., Laptrakoon, T., Tripetchkul, S. and Akeprathumchai, S. (2017). Utilization of Compost Tea from Coir Pith Compost in Parachute Rice Transplantation. In The 55th Kasetsart University Annual Conference. (pp. 919-926). Bangkok, Kasetsart University (in Thai)

Land Development Department. (2025, 10 January). Wonder PD. https://www.ldd.go.th/menu_5wonder/ (in Thai)

Li, H., Zhang, M., Zhang, Y., Xu, X., Zhao, Y., Jiang, X., Zhang, R. and Gui, Z. (2023). Characterization of Cellulose-Degrading Bacteria Isolated from Silkworm Excrement and Optimization of Its Cellulase Production. Polymers, 15(20), 4142. https://doi.org/10.3390/polym15204142

Liaotrakoon, W., Jirasatitanusorn, T., Aemnin, N. and Liaotrakoon, V. (2018). Effect of Drying Temperatures on Drying Rate, Physical and Antioxidative Properties of Sesbania javanica Mig. Flowers. RMUTI JOURNAL Science and Technology, 11(3), 57-69. https://ph01.tci-thaijo.org/index.php/rmutijo/article/view/167535/120747 (in Thai)

Liu, D., Chen, L., Zhu, X., Wang, Y., Xuan, Y., Liu X., Chen, L. and Duan, Y. (2018). Klebsiella pneumoniae SnebYK Mediates Resistance Against Heterodera glycines and Promotes Soybean Growth. Frontier in Microbiology, 9, 1134. https://doi.org/10.3389/fmicb.2018.01134

Muhammad, A., Fang, Y., Hou, Y. and Shi, Z. (2017). The Gut Entomotype of Red Palm Weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae) and Their Effect on Host Nutrition Metabolism. Frontiers in Microbiology, 8, 1-15. https://doi.org/10.3389/fmicb.2017.02291

ONT. (2022, 3 May). 16S Barcoding Kit. https://nanoporetech.com

Rattanaporn, K. (2019). Improvement of the Green Coconut Fiber Properties by Cellulase Enzyme for Yarn Production [Master's Thesis]. Rajamangala University of Technology Thanyaburi. http://www.repository.rmutt.ac.th/xmlui/handle/123456789/3848 (in Thai)

Ramsay, I.R. and Pullammanappallil, P.C. (2001). Protein Degradation During Anaerobic Wastewater Treatment: Derivation of Stoichiometry. Biodegradation, 12, 247-257. https://doi.org/10.1023/A:1013116728817

Refilda, Yasmine, S. and Zilfa. (2019). Characterization and Utilization of Young Coconut Waste (Cocos nucifera L) for Manufacturing Fermented Plant Extracts having Potential as Natural Fertilizer and Pesticide. Research of Journal Chemistry and Environment, 23(2), 23-30. http://repo.unand.ac.id/28383/1/BP-RFD-01.pdf

Rodrı́guez, H. and Fraga, R. (1999). Phosphate Solubilizing Bacteria and Their Role in Plant Growth Promotion. Biotechnology Advances, 17(4-5), 319-339. https://doi.org/10.1016/S0734-9750(99)00014-2

Ruengdechawiwat, S., Sanawong, P. and Thuanrod, O. (2022). Fermented Bioextracts Natural Products to Enhance Growth and Yield of RD41 Rice (Oryza sativa L.). Science and Technology Nakhon Sawan Rajabhat University Journal, 14(19), 1-11. https://ph02.tci-thaijo.org/index.php/JSTNSRU/article/view/244120/166904 (in Thai)

Saradhuldhat, P., Choorueng, M., Abdullakasim, S. and Phavaphutanon, L. (2014). Macro-Nutrient Content in Aromatic Coconut Produce. Khon Kaen Agriculture Journal, 42, 192-197. https://ag2.kku.ac.th/kaj/PDF.cfm?filename=F_037.pdf&id=1565&keeptrack=8 (in Thai)

Saxena, R.K., Sharmila, P. and Singh, V.P. (1995). Microbial Degradation of Tannins. Progress in Industrial Microbiology, 32, 259-270. https://doi.org/10.1016/S0079-6352(06)80038-X

Simionescu, N. and Petrovici, A.-R. (2024). Enhancing the Antioxidant Potential of Weissella confusa PP29 Probiotic Media through Incorporation of Hibiscus sabdariffa L. Anthocyanin Extract. Antioxidants, 13(2), 165. https://doi.org/10.3390/antiox13020165

Sirisangsawang, R. and Phetyim, N. (2023). Optimization of Tannin Extraction from Coconut Coir through Response Surface Methodology. Heliyon, 9(2), e13377. https://doi.org/10.1016/j.heliyon.2023.e13377

Song, P., Zhang, X., Wang, S., Xu, W., Wang, F., Fu, R. and Wei, F. (2023). Microbial Proteases and Their Applications. Frontier in Microbiology, 14, 1236368. https://doi.org/10.3389/fmicb.2023.1236368

Sukkhee, N., Vinitnantharat, S., Pratinthong, N., Intarapong, P., Singtosri, S. and Manakij, A. (2024). Organic Plant Pot from Discarded Aromatic Coconut and Flooring Materials from Pig House. Science and Technology Nakhon Sawan Rajabhat University Journal, 16(24), 42-55. https://ph02.tci-thaijo.org/index.php/JSTNSRU/article/view/253448/171305 (in Thai)

Sungtong, S., Pengnoo, A. and Boonyapipat, P. (2021). Efficacy of Bacillus spp. in Controlling Soilborne Pathogen Rigidoporus microporus under Control Conditions. Songklanakarin Journal of Plant Science, 8(1), 34-43. https://www.sjplantscience.com/index.php/ojs/article/view/48/23 (in Thai)

Tomás-Cortázar, J., Plaza-Vinuesa, L., de las Rivas, B., Lavin, J.L., Barriales, D., Abecia, L., Mancheno, J.M., Aransay, A.M., Munoz, R., Anguita, J. and Rodriguez, H. (2018). Identification of a Highly Active Tannase Enzyme from the Oral Pathogen Fusobacterium nucleatum subsp. Polymorphum. Microbial Cell Factories, 17(33), https://doi.org/10.1186/s12934-018-0880-4

Vaithanomsat, P., Apiwatanapiwat, W., Chumchuent, N., Kongtud, W. and Sundhrarajun, S. (2011). The Potential of Coconut Husk Utilization for Bioethanol Production. Kasetsart Journal - Natural Science, 45(1), 159-164.

Wattana, K., Pankrun, R. and Sriplai, A. (2022). The Effect of Various Fermented Bio-extract on Yield of Thai Striped Eggplant (Solanum melongena L.) Variety Nang Pha Ya, Agriculture and Technology Journal, 3(2), 76-85. https://li01.tci-thaijo.org/index.php/atj/article/view/255339/177895 (in Thai)

Yang, F., Chen, C., Ni, D., Yang, Y., Tian, J., Li, Y., Chen, S., Ye, X. and Wang, L. (2023). Effects of Fermentation on Bioactivity and the Composition of Polyphenols Contained in Polyphenol-Rich Foods: A Review. Foods, 12(17), 3315. https://doi.org/10.3390/foods12173315

Yu, A.O., Leveau, J.H.J. and Marco, M.L. (2020). Abundance, Diversity and Plant-Specific Adaptations of Plant-associated Lactic Acid Bacteria. Environmental Microbiology Reports, 12(1), 16-29. https://doi.org/10.1111/1758-2229.12794

Zhu, M., Xu, D., Liao, C., Zhang, T., Zhou, B., Wang, K., Li, P., Cheng, Z. and Chen, C. (2024). Microbial Dynamics and Pathogen Control During Fermentation of Distiller Grains: Effects of Fermentation Time on Feed Safety. International Journal of Molecular Sciences, 25(21), 11463. https://doi.org/10.3390/ijms252111463