DNA Fingerprint Study of Pigs Using RAPD-PCR Technique

Abstract

DNA fingerprint studys of 4 species of pigs from the Research and Training Phusing Center, Rajamangala University of Technology Isan, Kalasin Campus: 1) Large white; 2) Landrace; 3) Duroc-Jersey; and 4) Meishan were used. DNA were extracted from blood and absorbed 1.67, 1.76,1.50 and 1.53, respectively. DNA samples obtained from RAPD-PCR technique with primer oligo 101 (5' to 3' sequence GCG CCT GGA G) were isolated 1.5 percent (W/V) agarose gel electrophoresis, 100 volt for 30 mins. Gels were stained with ethidium bromide for 5 mills, rinsed for 10 mins. DNA fragments were determined by UV-Transilluminator, photographed and compared with lamda hind marker and 100 base pair marker. In this research, DNA patterns were 90 bp of Large white, 2,000 bp of Landrace, 70 bp of Duroc-Jersey and 450 bp of Meishan, respectively. For our results, DNA patterns were used to study preliminary genetic diversity for improving and developing each pig species.