อิทธิพลของพลาสมาเย็นต่อเซลล์มะเร็งในโครงเลี้ยงเซลล์แบบสามมิติจากวัสดุชีวภาพ Impact of Plasma Treatment on Cancer Cell Culture in 3D Biomaterial Scaffold

Abstract

Cancer is one of three major causes of death around the world. There are several techniques for cancer treatment. Nevertheless, most current treatments cause severe side effects to the patient. Recently, Cold Plasma technology has been introduced and implemented as a tumor and cancer therapy in mammalian cells. Several studies showed the benefit of Cold Plasma on cancer cell death induction without damaging neighboring normal cells. However, the Cold Plasma treatment conditions such as plasma power, gas, and time for properly treat the infected cell has not been clearly defined. In this study, investigation of Cold Plasma treatment on cancer cell will be performed based on tissue engineering approach. Three dimension scaffold plays an important role on the structure and biological mimic condition for cell adhesion and proliferation. This biomaterial 3D scaffold was fabricated from fibroin and chitosan using freeze drying technique, which then will be cultured with chondrosarcomar cells (cartilage cancer cells) incubated at 37ºC 5% CO₂ for 1 day for the investigation. Then, the treatment of Cold Plasma with various conditions of electrical plasma power, percentage of O₂ mixed gas, and duration will be investigated based on Factorial Experimental Design. Consequently, the survival cell percentage  after Cold Plasma treatments will be determined by MTT assay. Finally, the results of survival cell percentage show that the condition of 30 s. treatment time, 30 ml/min O₂ with Argon and 30 Watt power causes the lowest cell viability of 83.33 percentage