Separation of Cellulase and Reducing Sugar from Napier Grass Hydrolyzate by Membrane Filtration

Main Article Content

Mintita Pichadpongwimut
Thirawat Mueansichai
Juraivan Ratanapisit

Abstract

This study investigated separation of cellulase by ultrafiltration (UF) and reducing sugar by nanofiltration (NF) from the Napier grass hydrolyzate. Experiments were consisted of 5 membranes with different molecular weight cut-off (MWCO): PES500, PES100, PES10 for UF and NP010, NP030 for NF. The UF cross-flow study was carried out at 3 flow rates (30, 50 and 75 ml/min). Permeate from the UF was used as a feed to a dead-end NF which was operated for 4 transmembrane pressure (TMP) (10, 15, 20 and 25 bar). Results showed that the permeate flux was significantly related to the flow rate and TMP. The PES100 yielded the best flux for UF at 75 ml/min. Also the rejection of cellulase, reducing sugar and ethanol in both UF and NF significantly depended on MWCO. Fouling by cake layer formation provided the highest R2 based on the Hermia fouling model. The irreversible fouling (IF) of PES100 was the lowest (14.3%) at the low flow rate (30 ml/min) which related to the declining of the permeate flux. In addition, the IF was influenced by the MWCO and TMP in the limit of higher feed flow rate.

Article Details

How to Cite
Pichadpongwimut, M., Mueansichai, T., & Ratanapisit, J. (2019). Separation of Cellulase and Reducing Sugar from Napier Grass Hydrolyzate by Membrane Filtration. Frontiers in Engineering Innovation Research, 17(2), 1–11. Retrieved from https://ph01.tci-thaijo.org/index.php/jermutt/article/view/241897
Section
Research Articles

References

Gnansounou E, Dauriat A. Techno-economic analysis of lignocellulosic ethanol: review. Bioresource Technology. 2010;101:4980-4991.

Gomes DG, Serna LS, Cardona AC,Gama M, Domingues L. Insights into the economic viability of cellulases recycling on bioethanol production from recycled paper sludge. Bioresource Technology.2018;267:347-355.

Qi B, Chen X, Su Y, Wan Y. Enzyme adsorption and recycling during hydrolysis of wheat straw lignocellulose. Bioresource Technology. 2011;102: 2881-2889.

Khoshnevisan K, Vakhshiteh F, Barkhi M, Baharifare H, Poor AE, Zari N, Stamatis H, Bordbar AK. Immobilization of cellulase enzyme onto magnetic nanoparticles: Applications and recent advances. Molecular Catalysis. 2017;442: 66-73.

Chen G, Song W, Qi B, Lu J, Wan Y. Recycling cellulase from enzymatic hydrolyzate of acid treated wheat straw by electroultrafiltration.Bioresource Technology. 2013;144: 186-193.

Qi B, Luo J, Chen G, Chen X, Wan Y. Application of ultrafiltration and nanofiltration for recycling cellulase and concentrating glucose from enzymatic hydrolyzate of steam exploded wheat straw. Bioresoure Technology. 2012;104:466-472.

Charcosset C. Membrane process in biotechnology: An overview. Biotechnology Advances. 2006;24:482- 492.

Saxena A, Tripathi BP, Kumar M, Shahi VK. Membrane based techniques for the separation and purification of proteins: An overview. Advances in Colloid and Interface Science. 2009;145:1-22.

Strathmann H. Membrane process in biotechnology. Trend in Biotechnology. 1985;3(5):112-118.

Jutakanoke R, Leepipatpiboon N, Tolieng V, Kitpreechavanich V,Srinorakutara T, Akaracharanya A. Sugarcane leaves: pretreatment and ethanol fermentation by saccharomyces cerevisiae. Biomass and Bioenergy. 2012;39:283-289.

Yang S, Ding W, Chen H. Enzymatic hydrolysis of corn stalk in a hollow fiber ultrafiltration membrane reactor. Biomass and Bioenergy. 2009;33: 332-336.

Goulas AK, Kapasakalidis PG, Sinclair HR, Rastall RA, Grandison AS. Purification of oligosaccharides by nanofiltration. Journal of Membrane Science. 2002;209(1):321-335.

Qi B, Luo J, Chen X, Hang X, Wan Y. Separation of furfural from monosaccharides by nanofiltration. Bioresource Technology. 2011;102 (14):7111-7118.

Nurul I, Amin HM, Mohammad AW, Markom M, Peng LC, Hilal N. Analysis of deposition mechanism during ultrafiltration of glycerin-rich solutions. Desalination. 2010; 261:313-320.

Kanani DM, Sun X, Ghosh R. Reversible and irreversible membrane fouling during in-line microfiltration of concentrated protein solutions. Journal of Membrane Science. 2008;315:1-10.

Phitsuwan P, Sakka K, Ratanakhanokchai K. Structural changes and enzymatic response of Napier grass (Pennisetum purpureum) stem induced by alkaline pretreatment. Bioresource Technology. 2016;218: 247-256.

Nguyen N, Fargues C, Guiga W, Lameloise ML. Assessing nanofiltration and reverse osmosis for the detoxification of lignocellulosic hydrolysates. Journal of Membrane Science. 2015;487:40-50.

Jacques H. Constant pressure blocking filtration laws. Application to power-law non Newtonian fluid. Trans. Inst. Chem. Eng. Lond. 1982;60:183–187.

Alcaina CC, Blanco SÁ, Bes-Piá MA, Mendoza-Roca JA, Pastor-Alcañiz L. Ultrafiltration of residual fermentation brines from the production of table olives at different operating conditions. Journal of Cleaner Production. 2018;189: 662- 672.

Carbonell-Alcaina C, Corbatón-Báguena MJ, Álvarez-Blanco S, Bes-Piá MA, Mendoza-RocaJA, Pastor-Alcañiz L. Determination of fouling mechanisms in polymeric ultrafiltration membranes using residual brines from table olive storage wastewaters as feed. Journal of Food Engineering. 2016;187:14-23.

Vikbjerg AF, Jonsson G, Mu H, Xu X. Application of ultrafiltration membranes for purification of structured phosphorlipids produced by lipasecatalyzed acidolysis. Separation and Purification Technology. 2006;50: 184-191.

Asif MB, Hai FI, Dhar BR, Ngo HH, Guo W, Jegatheesan V, Price WE, Nghiem LD, Yamamoto K. Impact of simultaneous retention of micro pollutants and laccase on micro pollutant degradation in enzymatic membrane bioreactor. Bioresource Technology. 2018;267:473-480.

Guo S, Luo J, Wu Y, Qi B, Chen X, Wan Y. Decoloration of sugarcane molasses by tight ultrafiltration: Filtration behavior and fouling control. Separation and Purification Technology. 2018;204:66-74.

Kumar SM, Roy S. Filtration characteristics in dead-end microfiltration of living Saccharomyces cerevisiae cells by alumina membranes. Desalination. 2008;229:348–361.