Separation of Cellulase and Reducing Sugar from Napier Grass Hydrolyzate by Membrane Filtration

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Mintita Pichadpongwimut
Thirawat Mueansichai
Juraivan Ratanapisit


This study investigated separation of cellulase by ultrafiltration (UF) and reducing sugar by nanofiltration (NF) from the Napier grass hydrolyzate. Experiments were consisted of 5 membranes with different molecular weight cut-off (MWCO): PES500, PES100, PES10 for UF and NP010, NP030 for NF. The UF cross-flow study was carried out at 3 flow rates (30, 50 and 75 ml/min). Permeate from the UF was used as a feed to a dead-end NF which was operated for 4 transmembrane pressure (TMP) (10, 15, 20 and 25 bar). Results showed that the permeate flux was significantly related to the flow rate and TMP. The PES100 yielded the best flux for UF at 75 ml/min. Also the rejection of cellulase, reducing sugar and ethanol in both UF and NF significantly depended on MWCO. Fouling by cake layer formation provided the highest R2 based on the Hermia fouling model. The irreversible fouling (IF) of PES100 was the lowest (14.3%) at the low flow rate (30 ml/min) which related to the declining of the permeate flux. In addition, the IF was influenced by the MWCO and TMP in the limit of higher feed flow rate.

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Pichadpongwimut, M., Mueansichai, T., & Ratanapisit, J. (2019). Separation of Cellulase and Reducing Sugar from Napier Grass Hydrolyzate by Membrane Filtration. Journal of Engineering, RMUTT, 17(2), 1–11. Retrieved from
Research Articles


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