Automated Dehydration Assessment in Cannabis via YOLOv8 Localization and Multi-Modal Feature Classification

Main Article Content

Nittaya Muangnak
Wattana Pongnangchai
Natakorn Thasnas
Panida Songram
Nattapon Chapradit

Abstract

Water management is a fundamental aspect of plant physiology, directly influencing photosynthesis and nutrient intake. The objective of this study is to develop and validate a non-invasive, automated pipeline for quantifying water stress in Cannabis sativa L. using computer vision and physiological ground-truth measurements. The study utilized a dataset of 988 original outdoor images, which were expanded to 4,940 images through data augmentation to ensure model robustness. The process integrates the state-of-the-art YOLOv8 model for leaf localization, achieving a mean Average Precision (mAP) of 98.3 percent. A multi-modal feature set was constructed by combining color statistics derived from four color space transformations (RGB, HSV, LAB, and YCrCb) and Gray-Level Co-occurrence Matrix (GLCM) texture descriptors. The features were used to train a classifier to categorize dehydration into six severity levels (Normal, Mild, Moderate, Distinct, Severe, and Extreme), and the classifier was verified against ground-truth leaf water potential (Ψleaf) measurements. The nd- ings show that a Cubic Support Vector Machine (SVM) outperformed Artificial Neural Networks, achieving an overall accuracy of 86.2%. The research provides a scalable, high-precision solution for real-time monitoring in precision agriculture.

Article Details

How to Cite
[1]
N. Muangnak, W. Pongnangchai, N. Thasnas, P. Songram, and N. Chapradit, “Automated Dehydration Assessment in Cannabis via YOLOv8 Localization and Multi-Modal Feature Classification”, ECTI-CIT Transactions, vol. 20, no. 3, pp. 462–474, Jun. 2026.
Section
Research Article

References

B. Wu et al., “Quantifying global agricultural water appropriation with data derived from earth observations,” Journal of Cleaner Production, vol. 358, p. 131891, Jul. 2022.

V. Masson-Delmotte et al., Eds., Climate Change 2021: The Physical Science Basis. Contribution of Working Group I to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge, United Kingdom and New York, NY, USA: Cambridge University Press, 2021.

J. Flexas, J. Bota, J. M. Escalona, B. Sampol and H. Medrano, “Photosynthesis limitations during water stress acclimation and recovery in the drought-adapted Vitis hybrid Richter-110,” Journal of Experimental Botany, vol. 60, no. 8, pp. 2361–2377, 2009.

B. Madan, A. Malik and N. Raghuram, Crop nitrogen use efficiency for sustainable food security and climate change mitigation, 2022.

L. Sueree, F. Madaka, S. Jongrungruangchok, N. Vipunngeun and T. Songsak, “Comprehensive Pharmacognostic Analysis and Quality Control Parameters of Cannabis sativa L. subsp. sativa: Evaluating Leaves and Flowers for Medicinal Applications,” ASEAN Journal of Scientific and Technological Reports, vol. 28, no. 6, p. e259515, Oct. 2025.

I. Trancoso et al., “Cannabis sativa L.: Crop Management and Abiotic Factors That Affect Phytocannabinoid Production,” Agronomy, vol. 12, no. 7, p. 1492, 2022.

S. Datta and S. Taghvaeian, “Soil water sensors for irrigation scheduling in the United States: A systematic review of literature,” Agricultural Water Management, vol. 278, p. 108148, Mar. 2023.

C. G. Christenson, M. R. Gohardoust, S. Calleja, K. R. Thorp, M. Tuller and D. Pauli, “Monitoring cotton water status with microtensiometers,” Irrig Sci, vol. 42, no. 5, pp. 995–1011, Sep. 2024.

A. Yadav, H. Upreti and G. D. Singhal, “Crop water stress index and its sensitivity to meteorological parameters and canopy temperature,” Theor Appl Climatol, vol. 155, no. 4, pp. 2903–2915, Apr. 2024.

R. P. Sishodia, R. L. Ray and S. K. Singh, “Applications of Remote Sensing in Precision Agriculture: A Review,” Remote Sensing, vol. 12, no. 19, p. 3136, Jan. 2020.

A. V. Panchal, S. C. Patel, K. Bagyalakshmi, P. Kumar, I. R. Khan and M. Soni, “Image-based Plant Diseases Detection using Deep Learning,” Materials Today: Proceedings, vol. 80, pp. 3500–3506, Jan. 2023.

Ultralytics, “Explore Ultralytics YOLOv8,” Accessed: Apr. 12, 2026.

G. Estavillo et al., “Optimization of photosynthetic productivity in contrasting environments by regulons controlling plant form and function,” Int. J. Mol. Sci., vol. 19, no. 3, p. 872, Mar. 2018.

“Physicochemical environment,” in Fundamentals of Tropical Freshwater Wetlands, Elsevier, 2022, pp. 87–109.

S. Pooja and A. Singh, “The physiology of plant responses to drought,” Science, vol. 368, no. 6488, pp. 266–269, Apr. 2020.

S. I. Zandalinas and R. Mittler, “Plant responses to multifactorial stress combination,” New Phytologist, vol. 234, no. 4, pp. 1161–1167, 2022.

O. Rys et al., “Photosynthetic metabolism under stressful growth conditions as a basis for crop breeding and yield improvement,” Plants, vol. 9, no. 1, p. 88, Jan. 2020.

U. Yousuf, A. Ganie, I. Khan, A. Qureshi and M. Khan, “Plant water relations,” in Plant Life under Changing Environment, D. Tripathi, P. Singh, S. Chauhan, Eds. Academic Press, 2020, pp. 87–109.

C. M. Rodriguez-Dominguez et al., “Leaf water potential measurements using the pressure chamber: Synthetic testing of assumptions towards best practices for precision and accuracy,” Plant, Cell & Environment, vol. 45, no. 7, pp. 2037–2061, 2022.

R. B. Thompson, M. Gallardo, L. C. Valdez and M. D. Fern´andez, “Using plant water status to define threshold values for irrigation management of vegetable crops using soil moisture sensors,” Agricultural Water Management, vol. 88, no. 1, pp. 147–158, Mar. 2007.

S. G. Kanade and J. P. S. Chauhan, “Greenness identification using visible spectral colour indices for site specific weed management,” Plant Physiol. Rep., vol. 26, no. 1, pp. 114–125, Mar. 2021.

T. Gill, S. K. Gill, D. K. Saini, Y. Chopra, J. P. de Koff and K. S. Sandhu, “A Comprehensive Review of High Throughput Phenotyping and Machine Learning for Plant Stress Phenotyping,” Phenomics, vol. 2, no. 3, pp. 156–183, Jun. 2022.

Anjna, M. Sood, and P. K. Singh, “Hybrid System for Detection and Classification of Plant Disease Using Qualitative Texture Features Analysis,” Procedia Computer Science, vol. 167, pp. 1056–1065, Jan. 2020.

K. Neupane and F. Baysal-Gurel, “Automatic Identification and Monitoring of Plant Diseases Using Unmanned Aerial Vehicles: A Review,” Remote Sensing, vol. 13, no. 19, p. 3841, Jan. 2021.

J. Peguero-Pina et al., “Photochemical reflectance index (PRI) for detecting responses of diurnal and seasonal photosynthetic activity to experimental drought and warming in a Mediterranean shrubland,” Remote Sens., vol. 9, no. 11, p. 1189, Nov. 2017.

S. S. Chouhan, U. P. Singh and S. Jain, “Image processing and pattern recognition based plant leaf diseases identification and classification,” J. Phys.: Conf. Ser., vol. 1804, no. 1, p. 012160, Feb. 2021.

J.-F. Zhang, C.-E. Lee, C. Liu, Y. S. Shao, S. W. Keckler and Z. Zhang, “SNAP: An Efficient Sparse Neural Acceleration Processor for Unstructured Sparse Deep Neural Network Inference,” IEEE Journal of Solid-State Circuits, vol. 56, no. 2, pp. 636–647, Feb. 2021.

X. Gong and S. Zhang, “A High-Precision Detection Method of Apple Leaf Diseases Using Improved Faster R-CNN,” Agriculture, vol. 13, no. 2, p. 240, Feb. 2023.

Z. Zhang et al., “RPH-Counter: Field detection and counting of rice planthoppers using a fully convolutional network with object-level supervision,” Computers and Electronics in Agriculture, vol. 225, p. 109242, Oct. 2024.

J. Yao, S. N. Tran, S. Garg and S. Sawyer, “Deep Learning for Plant Identification and Disease Classification from Leaf Images: Multiprediction Approaches,” ACM Comput. Surv., vol. 56, no. 6, pp. 153:1-153:37, Feb. 2024.

Y. Han et al., “Calibration and Image Processing of Aerial Thermal Image for UAV Application in Crop Water Stress Estimation,” Journal of Sensors, vol. 2021, no. 1, p. 5537795, 2021.

N. Chaachouaya, A. Azerouala, B. Bencharkia, A. Douirab and L. Zidaneb, “Cannabis sativa L.: A Review on Traditional Uses, Botany, Phytochemistry, and Pharmacological Aspects,” Traditional and Integrative Medicine, Apr. 2023.

S. Sharma et al., “The Effects of Water-Deficit Stress on Cannabis sativa L. Development and Production of Secondary Metabolites: A Review,” Horticulturae, vol. 11, no. 6, p. 646, Jun. 2025.

R. Kalayasiri and S. Boonthae, “Trends of cannabis use and related harms before and after legalization for recreational purposes in a developing country in Asia,” BMC Public Health, vol. 23, no. 1, p. 911, May 2023.

J. S. Boyer, “Leaf Water Potentials Measured with a Pressure Chamber,” Plant Physiol, vol. 42, no. 1, pp. 133–137, Jan. 1967.

Z. Zheng et al., “Enhancing Geometric Factors in Model Learning and Inference for Object Detection and Instance Segmentation,” in IEEE Transactions on Cybernetics, vol. 52, no. 8, pp. 8574-8586, Aug. 2022

P. Jiang, Y. Chen, B. Liu, D. He and C. Liang, “Real-Time Detection of Apple Leaf Diseases Using Deep Learning Approach Based on Improved Convolutional Neural Networks,” in IEEE Access, vol. 7, pp. 59069-59080, 2019

S. Ghosal et al., “A Weakly Supervised Deep Learning Framework for Sorghum Head Detection and Counting,” Plant Phenomics, vol. 2019, Jun. 2019.

J. Liu and X. Wang, “Tomato Diseases and Pests Detection Based on Improved Yolo V3 Convolutional Neural Network,” Front. Plant Sci., vol. 11, Jun. 2020.